C5b-9 antibody

Catalog No. ABIN8065530

The Mouse Monoclonal anti-C5b-9 antibody is suitable to detect C5b-9 in samples from Human. It has been validated for ELISA.

Quick Overview for C5b-9 antibody (ABIN8065530)

Target: C5b-9 (Terminal Complement Complex C5b-9 (C5b-9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This C5b-9 antibody is un-conjugated

Application: ELISA

Grade: Carrier-free

Clone: AE11

Product Details anti-C5b-9 Antibody

Purpose: Anti-Complement C5b-9 Antibody

Specificity: DIA 011-01 binds both membrane-bound MAC (active) and fluid-phase SC5b-9 complexes (inactive). (1) DIA 011-01 binds to a neoepitope exposed on C9

Predicted Reactivity: Horse,Human,Non-Human Primate,and Pig

Purification: Protein A or Protein G purified

Immunogen: Purified C5b-9

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C without exposure to light

Expiry Date: 12 months

Target Details for C5b-9

Target: C5b-9 (Terminal Complement Complex C5b-9 (C5b-9))

Alternative Name: Complement C5b-9

Background: C5, C5D, C5a, C5b, CPAMD4, ECLZB, complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, C5a anaphylatoxin, anaphylatoxin C5a analog, complement component 5, prepro-C5,Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed:12878586, PubMed:18204047, PubMed:30643019, PubMed:6554279). Activated downstream of classical, alternative, lectin and GZMK complement pathways (PubMed:12878586, PubMed:18204047, PubMed:30643019, PubMed:39914456, PubMed:39814882, PubMed:6554279). Complement C5b Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed:26841837, PubMed:27052168, PubMed:30552328, PubMed:30643019). Complement C5b is generated following cleavage by C5 convertase and initiates formation of the MAC complex: C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9 (PubMed:30552328, PubMed:30643019). During MAC complex assembly, the C5b6 subcomplex, composed of complement C5b and C6, associates with the outer leaflet of target cell membrane, reducing the energy for membrane bending (PubMed:30552328, PubMed:32569291).,C5, C6, C7, C8, C9,C5b-9 is also known as the terminal complement complex (TCC). The TCC consists of C5b, C6, C7, C8 and C9 and forms the membrane attack complex (MAC) as well as the non-lytic fluid-phase SC5b-9 complex (with protein S). The MAC forms channels in target cell membranes leading to cell lysis by osmotic leakage. The complexes contain neoantigens that are absent from the individual native components from which they are formed and DIA 011-01 is directed against a neoepitope exposed on C9 when incorporated into the TCC.

Gene ID: 727

UniProt: P01031, P13671, P06681, P07357, P07358, P07360, P02749