C1S antibody

Catalog No. ABIN8065539

The Mouse Monoclonal anti-C1S antibody is suitable to detect C1S in samples from Human. It has been validated for ELISA and IF.

Quick Overview for C1S antibody (ABIN8065539)

Target: C1S (Complement Component 1, S Subcomponent (C1S))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This C1S antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Grade: Carrier-free

Clone: 49

Product Details anti-C1S Antibody

Purpose: Anti-Pro/Actived Complement C1s Antibody

Specificity: ABS 002-49 is specific for C1s proenzyme and activated free C1s and C1s in complex with C1 inhibitor. Epitope differs from that of ABS 002-09.

Predicted Reactivity: Human

Purification: Protein A or Protein G purified

Immunogen: Human plasma C1s Proenzyme

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C without exposure to light

Expiry Date: 24 months

Target Details for C1S

Target: C1S (Complement Component 1, S Subcomponent (C1S))

Alternative Name: Pro/Actived Complement C1s

Background: C1S, EDSPD2, complement C1s subcomponent, C1 esterase, basic proline-rich peptide IB-1, complement component 1 subcomponent s, complement component 1, s subcomponent,Component of the complement C1 complex, a multiprotein complex that initiates the classical pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed:11445589, PubMed:16169853, PubMed:417728, PubMed:467643, PubMed:6271784, PubMed:6282646, PubMed:6319179, PubMed:70787, PubMed:9422791). C1S is activated following association of the C1 complex with immunoglobulins (IgG or IgM) complexed with antigens to form antigen-antibody complexes on the surface of pathogens (PubMed:34155115). C1S is cleaved and activated by C1R to generate C1s subcomponent heavy and light chains (PubMed:11445589, PubMed:6271784). C1s subcomponent light chain then cleaves and activates C2 and C4, the next components of the classical complement pathway (PubMed:16169853, PubMed:467643, PubMed:6282646, PubMed:6319179, PubMed:6906228, PubMed:70787, PubMed:9422791). {ECO:0000269|PubMed:11445589, ECO:0000269|PubMed:16169853, ECO:0000269|PubMed:34155115, ECO:0000269|PubMed:417728, ECO:0000269|PubMed:467643, ECO:0000269|PubMed:6271784, ECO:0000269|PubMed:6282646, ECO:0000269|PubMed:6319179, ECO:0000269|PubMed:6906228, ECO:0000269|PubMed:70787, ECO:0000269|PubMed:9422791}., [Complement C1s subcomponent light chain]: Serine protease component of the complement C1 complex, which catalyzes cleavage and activation of C2 and C4, the next components of the classical complement pathway (PubMed:16169853, PubMed:22949645, PubMed:417728, PubMed:467643, PubMed:6282646, PubMed:6319179, PubMed:70787, PubMed:9422791). Also able to cleave C1 inhibitor (SERPING1) in vitro, additional evidence is however required to confirm this result in vivo (PubMed:16169853). Also cleaves IGFBP5 and thereby inhibits the trophic effects of IGF1 (PubMed:18930415). {ECO:0000269|PubMed:16169853, ECO:0000269|PubMed:18930415, ECO:0000269|PubMed:22949645, ECO:0000269|PubMed:417728, ECO:0000269|PubMed:467643, ECO:0000269|PubMed:6282646, ECO:0000269|PubMed:6319179, ECO:0000269|PubMed:70787, ECO:0000269|PubMed:9422791}.,C1S,C1s proenzyme is a 79.8 kDa glycoprotein produced primarily by hepatocytes but also by other cells. The proenzyme is produced as a single-chain polypeptide of 688 aa. Upon activation the peptide is cleaved between R437 and I438 giving the activated enzyme consisting of two disulfide-linked chains, A chain (52.2 kDa) and B chain (27.7 kDa). The Ca-dependent catalytic subunit of the C1 complex (C1q, C1r, C1s) comprises of two C1s and two C1r molecules and this serine protease tetramer is responsible for the initial steps in the classical complement pathway, cleavage of C4 and C2.

Molecular Weight: Pro-enzyme C1s (pro-C1s) appears as a single-chain polypeptide around 80 kDa. Upon activation, it is cleaved into two disulfide-linked chains: a ~57 kDa A-chain and and 27 kDa B-chain).

Gene ID: 716

UniProt: P09871

Pathways: Complement System