ATG9A antibody (pSer735) (HRP)

Catalog No. ABIN756316

This Rabbit Polyclonal antibody specifically detects ATG9A in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Rat.

Quick Overview for ATG9A antibody (pSer735) (HRP) (ABIN756316)

Target: ATG9A (ATG9 Autophagy Related 9 Homolog A (S. Cerevisiae) (ATG9A))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG9A antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ATG9A Antibody (HRP)

Binding Specificity: pSer735

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human ATG9A around the phosphorylation site of Ser735

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ATG9A

Target: ATG9A (ATG9 Autophagy Related 9 Homolog A (S. Cerevisiae) (ATG9A))

Alternative Name: ATG9A

Background:

Synonyms: mATG9, APG9L1, MGD3208, Autophagy-related protein 9A, APG9-like 1, ATG9A

Background: Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H.

Gene ID: 79065

UniProt: Q7Z3C6