Separase antibody (pSer1073) (Biotin)

Catalog No. ABIN757869

This anti-Separase antibody is a Rabbit Polyclonal antibody detecting Separase in ELISA, IHC (p) and IHC (fro). Suitable for Human.

Quick Overview for Separase antibody (pSer1073) (Biotin) (ABIN757869)

Target: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Separase antibody is conjugated to Biotin

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Separase Antibody (Biotin)

Binding Specificity: pSer1073

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human ESPL1 around the phosphorylation site of Ser1073

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for Separase

Target: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Alternative Name: Separase

Background:

Synonyms: Caspase like protein ESPL1, ESP 1, ESP1, ESP-1, ESPL 1, ESPL1, ESPL-1, Extra spindle poles like 1, Extra spindle poles like 1 protein, Separin, Similar to fission yeast cut1and gene, SSE, Separase, ESPL1_HUMAN.

Background: Separase is a cysteine protease that is essential for mitotic progression by separating sister chromatids. Each cell must receive one chromatid of every chromosome, during mitosis. Cohesin plays an important role in cohering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys cohesin by means of cleavage, allowing the chromatids to separate and divide with the cell. Separase activity is highly regulated. It not only cleaves cohesin at the onset of anaphase but also cleaves itself, promoting downregulation of separase after anaphase. Should a human cell become an aneuploid (one too many or too few chromatids), the embryo most likely will not survive. Should the embryo survive, it will most likely develop severe birth defects or later develop malignant cancers.

Gene ID: 9700