Recombinant ACPP antibody (full length)

Catalog No. ABIN8092990

This Mouse Monoclonal antibody specifically detects ACPP in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant ACPP antibody (full length) (ABIN8092990)

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACPP antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-452M

Product Details anti-ACPP Antibody

Binding Specificity: full length

Purpose: ACP3 Antibody / Prostatic acid phosphatase

Immunogen: Recombinant full-length human ACPP3/PAP protein was used as the immunogen for the Prostatic Acid Phosphatase Antibody for IHC.

Isotype: IgG1, kappa

Application Details

Application Notes: 1. Optimal dilution of the Prostatic Acid Phosphatase Antibody for IHC should be determined by the researcher.

2. This ACP3/Prostatic acid phosphatase antibody is recombinantly produced by expression in CHO cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Prostatic Acid Phosphatase Antibody for IHC with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for ACPP

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Alternative Name: ACP3

Background: Prostatic acid phosphatase (ACP3), also known as PAP, is a secreted phosphatase highly expressed in prostate epithelium and widely used as a biomarker in prostate cancer research and pathology. Prostatic Acid Phosphatase Antibody for IHC (clone MSVA-452M) recognizes this prostate-associated enzyme, which belongs to the histidine acid phosphatase family and participates in phosphomonoester hydrolysis. PAP is predominantly expressed in prostate epithelial cells and secreted into prostatic fluid, where it contributes to normal prostate physiology and enzymatic activity within the gland.

Prostatic Acid Phosphatase Antibody for IHC enables visualization of PAP protein expression in formalin-fixed paraffin-embedded tissues using immunohistochemistry. In IHC staining, PAP is typically detected in prostate epithelial cells where staining may appear cytoplasmic or luminal due to the secretory nature of the enzyme. This staining pattern reflects the physiological localization of prostatic acid phosphatase in glandular epithelial cells of the prostate and related secretory tissues.

Immunohistochemistry detection of prostatic acid phosphatase has long been used in studies of prostate biology and prostate cancer. PAP expression is strongly associated with prostate epithelial differentiation and is frequently evaluated in prostate carcinoma research. IHC staining using Prostatic Acid Phosphatase Antibody for IHC allows researchers to identify PAP-positive prostate epithelial cells and examine expression patterns in prostate tumors and metastatic lesions.

Prostatic acid phosphatase was historically used as one of the earliest biomarkers for prostate cancer prior to the introduction of PSA testing, and it continues to serve as a valuable marker of prostate epithelial differentiation. PAP expression can be observed in prostate glandular epithelium and prostate carcinoma cells, supporting studies of tumor differentiation and prostate tissue biology.

Prostatic Acid Phosphatase Antibody for IHC provides a research tool for detecting PAP expression in prostate tissues and prostate cancer models. Immunohistochemistry staining of ACP3 enables investigators to examine prostate epithelial differentiation, evaluate tumor cell phenotype in prostate carcinoma, and study the biological role of ACP3 in prostate tissue physiology and prostate cancer progression.

This ACP3 antibody is also part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.

UniProt: P15309

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process