Recombinant CD166 antibody (full length)

Catalog No. ABIN8092993

This Mouse Monoclonal antibody specifically detects CD166 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD166 antibody (full length) (ABIN8092993)

Target: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD166 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RALCAM-9811

Product Details anti-CD166 Antibody

Binding Specificity: full length

Purpose: ALCAM Antibody / Activated leukocyte cell adhesion molecule

Immunogen: Recombinant full-length human CD166 protein was used as the immunogen for the ALCAM/Activated leukocyte cell adhesion molecule antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the ALCAM/Activated leukocyte cell adhesion molecule antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: ALCAM/Activated leukocyte cell adhesion molecule antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for CD166

Target: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Alternative Name: ALCAM

Background: ALCAM antibody targets Activated leukocyte cell adhesion molecule, a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily that mediates cell-cell adhesion through homophilic and heterophilic interactions. ALCAM is also widely known in the literature as CD166 and MEMD, and functions as an adhesion receptor involved in maintaining tissue architecture and regulating intercellular communication. The protein is primarily localized to the cell membrane, where it participates in stable cell-cell contacts and dynamic adhesive interactions in both immune and non-immune tissues.

Activated leukocyte cell adhesion molecule plays a critical role in immune cell trafficking, synapse formation, and leukocyte-endothelial interactions. Through its extracellular immunoglobulin-like domains, ALCAM mediates adhesion between activated leukocytes and other cell types, facilitating immune surveillance and inflammatory responses. In addition to immune contexts, ALCAM contributes to epithelial organization, neuronal development, and stem cell niche maintenance, reflecting its broad functional relevance across multiple biological systems.

ALCAM expression is observed in a variety of tissues, including hematopoietic cells, endothelial cells, epithelial cells, and neural tissues. At the cellular level, ALCAM antibody staining is typically membranous, consistent with its function as a surface adhesion molecule, although cytoplasmic staining may be observed depending on cellular context and protein turnover. The regulated expression of ALCAM is essential for balancing adhesive stability with cellular motility during development and tissue remodeling.

In cancer biology, ALCAM has been implicated in tumor progression, invasion, and metastasis, with expression levels and localization patterns varying across tumor types. Altered ALCAM-mediated adhesion can influence tumor cell cohesion, interactions with the tumor microenvironment, and metastatic dissemination. As a result, ALCAM expression has been explored as a marker of tumor differentiation, invasive potential, and cell adhesion status in several malignancies.

This ALCAM antibody is suitable for research applications aimed at detecting Activated leukocyte cell adhesion molecule expression and localization in cells and tissue sections. Clone rALCAM/9811 is designed to recognize ALCAM and supports investigation of cell adhesion mechanisms, immune cell interactions, and adhesion-related changes associated with disease states. Use of this antibody enables detailed study of CD166-associated signaling and structural functions in physiological and pathological models.

UniProt: Q13740