Recombinant CD166 antibody

Catalog No. ABIN8092996

This Mouse Monoclonal antibody specifically detects CD166 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD166 antibody (ABIN8092996)

Target: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD166 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Clone: RMOG-07

Product Details anti-CD166 Antibody

Purpose: ALCAM Antibody / CD166 (azide and preservative free)

Immunogen: The CD166/ALCAM antibody was raised against a 200 amino acid portion of human CD166.

Isotype: IgG2b, kappa

Application Details

Application Notes: 1. Optimal dilution of the CD166/ALCAM antibody should be determined by the researcher.

2. This CD166/ALCAM antibody is recombinantly produced by expression in CHO cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: CD166/ALCAM antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for CD166

Target: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Alternative Name: ALCAM

Background: CD166 antibody targets Activated leukocyte cell adhesion molecule, a cell surface glycoprotein encoded by the ALCAM gene and classified within the immunoglobulin superfamily of adhesion receptors. CD166, also referred to as ALCAM or melanoma-associated cell adhesion molecule, functions as a mediator of cell-cell adhesion through both homophilic CD166-CD166 interactions and heterophilic binding to CD6. This adhesive activity plays an important role in stabilizing intercellular contacts and regulating tissue organization in diverse biological contexts.

At the cellular level, CD166 is predominantly localized to the plasma membrane, where it contributes to the formation of adherens-like junctions and supports coordinated cell behavior. Expression of Activated leukocyte cell adhesion molecule is observed in epithelial cells, endothelial cells, neurons, and multiple immune cell populations, reflecting its broad involvement in developmental processes and immune regulation. CD166-mediated adhesion is particularly important in maintaining epithelial integrity and facilitating controlled cell migration during tissue remodeling.

CD166 has been extensively studied in cancer biology due to its association with tumor cell cohesion, invasion, and metastatic potential. Altered expression or redistribution of ALCAM can influence tumor architecture and interactions with the surrounding microenvironment. In several malignancies, changes in CD166 expression correlate with tumor differentiation status and disease progression, highlighting its relevance as a marker of adhesion dynamics rather than a lineage-restricted antigen.

This CD166 antibody is suitable for research applications involving detection of Activated leukocyte cell adhesion molecule expression and localization in cells and tissue sections. Clone rMOG/07 is designed to recognize CD166 and supports investigation of cell adhesion mechanisms, immune cell interactions, and adhesion-related alterations in physiological and pathological models. Use of this antibody enables focused study of ALCAM-associated structural and signaling roles across a range of experimental systems.

UniProt: Q13740