Recombinant ADAR antibody

Catalog No. ABIN8093000

The Rabbit Monoclonal anti-ADAR antibody is suitable to detect ADAR in samples from Human, Mouse and Rat. It has been validated for WB, IF and IHC (p).

Quick Overview for Recombinant ADAR antibody (ABIN8093000)

Target: ADAR (Adenosine Deaminase, RNA-Specific (ADAR))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ADAR antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AFCB-1

Product Details anti-ADAR Antibody

Purpose: ADAR Antibody / Adenosine deaminase acting on RNA

Purification: Purified

Immunogen: A synthesized peptide derived from human Adenosine deaminase acting on RNA protein was used as the immunogen for the ADAR antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ADAR antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the ADAR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ADAR

Target: ADAR (Adenosine Deaminase, RNA-Specific (ADAR))

Alternative Name: ADAR

Background: ADAR antibody targets Adenosine deaminase acting on RNA, an RNA-editing enzyme encoded by the ADAR gene that catalyzes the conversion of adenosine to inosine within double-stranded RNA substrates. This post-transcriptional modification alters RNA sequence information and can influence RNA stability, splicing, localization, and translation. Adenosine deaminase acting on RNA is a central regulator of RNA editing events that expand transcriptomic diversity and fine-tune gene expression across many cell types.

Adenosine deaminase acting on RNA contains conserved double-stranded RNA-binding domains at its N-terminus and a C-terminal deaminase catalytic domain. These domains enable selective binding to structured RNA regions and catalysis of deamination reactions. RNA editing mediated by ADAR can result in codon changes, modification of regulatory RNA elements, or alteration of microRNA processing. A short functional summary is that ADAR functions as a key enzymatic editor that modulates RNA sequence and function after transcription, thereby shaping protein diversity and regulatory RNA networks.

ADAR is broadly expressed in mammalian tissues, with particularly high expression reported in the brain, immune cells, and epithelial tissues. Subcellular localization is primarily nuclear, although cytoplasmic distribution has also been observed depending on cell type and activation state. Expression levels and activity of ADAR are dynamically regulated in response to developmental cues and cellular stress signals, including interferon signaling pathways, reflecting its role in innate immune regulation and RNA surveillance.

From a biological and disease relevance perspective, dysregulation of ADAR-mediated RNA editing has been linked to neurological disorders, autoimmune diseases, and cancer. Altered RNA editing patterns can disrupt normal gene function and contribute to aberrant signaling pathways. In immune contexts, ADAR activity helps distinguish self from non-self RNA, preventing inappropriate activation of antiviral responses. These diverse roles highlight the importance of precise control over ADAR expression and enzymatic activity in maintaining cellular homeostasis.

An ADAR antibody is a useful research tool for detecting adenosine deaminase acting on RNA expression and studying RNA editing mechanisms in cellular and tissue-based systems. Detection of ADAR supports investigations into RNA processing, transcriptome regulation, innate immune signaling, and disease-associated changes in RNA editing landscapes. This antibody targets adenosine deaminase acting on RNA for use in research applications focused on post-transcriptional gene regulation and RNA biology.

UniProt: P55265

Pathways: Protein targeting to Nucleus