Recombinant CALB1 antibody

Catalog No. ABIN8093023

This Mouse Monoclonal antibody specifically detects CALB1 in IHC and WB. It exhibits reactivity toward Human.

Quick Overview for Recombinant CALB1 antibody (ABIN8093023)

Target: CALB1 (Calbindin (CALB1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CALB1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: RCALB1-9805

Product Details anti-CALB1 Antibody

Purpose: Calbindin Antibody / CALB1

Immunogen: A recombinant fragment corresponding human CALB1 protein was used as the immunogen for the D28K/CALB1 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: 1. Optimal dilution of the D28K/CALB1 antibody should be determined by the researcher.

2. This D28K/CALB1 antibody is recombinantly produced by expression in CHO cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: CALB1/Vitamin D-dependent calcium-binding protein antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for CALB1

Target: CALB1 (Calbindin (CALB1))

Alternative Name: Calbindin

Background: D28K antibody, also known as CALB1 antibody, recognizes D28K, a widely used abbreviated name for the calcium-binding protein Calbindin 1 encoded by the CALB1 gene. The D28K designation reflects the approximate 28 kDa molecular size of this EF-hand calcium-binding protein and is commonly used in western blot figures, neuronal marker studies, and calcium signaling research. D28K functions as an intracellular calcium buffer that modulates the amplitude and kinetics of calcium transients, thereby shaping downstream signaling responses in excitable and secretory cells.

D28K is a member of the calbindin subfamily within the larger EF-hand protein family and contains six EF-hand motifs, four of which are capable of binding Ca2+ with high affinity. By reversibly binding calcium ions, D28K influences intracellular diffusion of calcium and limits cytotoxic calcium overload. This buffering activity is particularly important in cells exposed to rapid calcium flux, such as neurons and endocrine cells. Altered expression of CALB1 has been associated with dysregulated calcium homeostasis in neurodegenerative disorders, where reduced D28K levels may contribute to increased cellular susceptibility to stress and excitotoxic injury.

In the nervous system, D28K expression is frequently examined in the context of neuronal circuit mapping, interneuron characterization, and studies of synaptic plasticity. Outside the brain, CALB1 expression is detected in kidney distal tubular epithelium, intestinal absorptive cells, and pancreatic islets, where it supports calcium transport and hormone secretion processes. Because D28K is often cited in abbreviated form in primary research articles and figure legends, D28K antibody provides a convenient and literature-consistent terminology for investigators working with calcium-binding protein pathways. Clone rCALB1/9805 is a recombinant mouse monoclonal antibody generated through defined sequence expression, supporting lot-to-lot consistency and reproducibility in research applications focused on calcium regulation and cell type identification.

UniProt: P05937