Recombinant CPS1 antibody (full length)

Catalog No. ABIN8093029

This Rabbit Monoclonal antibody specifically detects CPS1 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Recombinant CPS1 antibody (full length) (ABIN8093029)

Target: CPS1 (Carbamoyl-Phosphate Synthase 1, Mitochondrial (CPS1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CPS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: CPS1-13326R

Product Details anti-CPS1 Antibody

Binding Specificity: full length

Purpose: CPS1 Antibody / Carbamoyl-phosphate synthase 1

Purification: Protein A affinity

Immunogen: Recombinant full-length human CPS1 protein was used as the immunogen for the Hepatocyte specific antigen/CPS1 antibody.

Isotype: IgG, kappa

Application Details

Application Notes: 1. Optimal dilution of the Hepatocyte specific antigen/CPS1 antibody should be determined by the researcher.

2. This Hepatocyte specific antigen/CPS1 antibody is recombinantly produced by expression in CHO cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Hepatocyte specific antigen/CPS1 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for CPS1

Target: CPS1 (Carbamoyl-Phosphate Synthase 1, Mitochondrial (CPS1))

Alternative Name: CPS1

Background: Hepatocyte specific antigen antibody targets Carbamoyl-phosphate synthase 1 (CPS1), a mitochondrial enzyme that catalyzes the first and rate-limiting step of the urea cycle. Carbamoyl-phosphate synthase 1 is responsible for converting ammonia and bicarbonate into carbamoyl phosphate within the mitochondrial matrix, a critical process for nitrogen detoxification in hepatocytes. Because CPS1 expression is highly enriched in liver parenchymal cells, it has long been referred to in diagnostic pathology as hepatocyte specific antigen and is widely used as a marker of hepatocellular differentiation.

Hepatocyte specific antigen antibody, also referred to as Carbamoyl-phosphate synthase 1 antibody and mitochondrial carbamoyl phosphate synthase antibody in the literature, highlights the strong metabolic specialization of hepatocytes. CPS1 is encoded by the CPS1 gene and is predominantly localized to mitochondria, producing a characteristic granular cytoplasmic staining pattern that reflects dense mitochondrial distribution in liver cells. This restricted localization and tissue specificity make CPS1 antibody a useful research tool for distinguishing hepatocyte-derived cells from non-hepatic lineages in complex tissue environments.

Carbamoyl-phosphate synthase 1 plays a central biological role in amino acid metabolism and ammonia detoxification. Disruption of CPS1 activity leads to urea cycle dysfunction and hyperammonemia, underscoring its essential metabolic function. Altered CPS1 expression has been reported in liver disease and hepatocellular carcinoma, often reflecting changes in hepatocyte differentiation state and mitochondrial function. Hepatocyte specific antigen antibody can therefore provide insight into hepatic metabolic competence without implying validation in any specific experimental application.

The CPS1 protein belongs to the class I amidoligase family and contains multiple conserved catalytic domains that coordinate ATP-dependent reactions. Its large size and mitochondrial targeting sequence contribute to its stable intracellular localization. Clone CPS1/9870 is designed to recognize Carbamoyl-phosphate synthase 1 and represents a distinct reagent for research use. A second mention of clone CPS1/9870 is included here to clearly associate this antibody with its defined clone identity for cataloging and database consistency.

In research settings, hepatocyte specific antigen antibody complements nuclear hepatocyte markers by providing metabolic and mitochondrial context rather than transcriptional identity alone. Because CPS1 is a functional enzyme rather than a regulatory protein, Carbamoyl-phosphate synthase 1 antibody is particularly useful for studies focused on hepatic metabolism, mitochondrial biology, and hepatocyte lineage characterization.

For a validated reference of CPS1 expression in liver and hepatocellular tumors, see the < a href=../tds/cps1-antibody-carbamoyl-phosphate-synthetase-1-cps19859-v5677>CPS1 antibody clone CPS1/9859 with supporting IHC and western blot data.

UniProt: P31327

Pathways: Response to Growth Hormone Stimulus, Cellular Glucan Metabolic Process