CXCR5 antibody

Catalog No. ABIN8093082

The Mouse Monoclonal anti-CXCR5 antibody (Clone CXCR5-13095) (ABIN8093082) specifically detects CXCR5 in IHC. The antibody is reactive with Human samples.

Quick Overview for CXCR5 antibody (ABIN8093082)

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CXCR5 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Grade: Carrier-free

Clone: CXCR5-13095

Product Details anti-CXCR5 Antibody

Purpose: CXCR5 Antibody / CD185 (azide and preservative free)

Immunogen: A recombinant fragment of human CXCR5 protein (exact sequence is proprietary) was used as the immunogen for the C-X-C Motif Chemokine Receptor 5/CD185 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the C-X-C Motif Chemokine Receptor 5/CD185 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: C-X-C Motif Chemokine Receptor 5/CD185 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for CXCR5

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Alternative Name: CXCR5

Background: C-X-C Motif Chemokine Receptor 5 antibody, also known as CD185 antibody, recognizes C-X-C motif chemokine receptor 5, a seven-transmembrane G protein-coupled receptor encoded by the CXCR5 gene and commonly referred to as Burkitt lymphoma receptor 1. C-X-C Motif Chemokine Receptor 5 is primarily localized to the plasma membrane of B lymphocytes and subsets of T cells, particularly follicular helper T cells, where it regulates chemokine-driven cell migration. As a member of the CXC chemokine receptor family, CXCR5 binds the ligand CXCL13 and plays a central role in lymphoid follicle organization and germinal center formation.

C-X-C Motif Chemokine Receptor 5 antibody detects a multi-pass transmembrane receptor composed of seven alpha-helical membrane-spanning domains, extracellular regions responsible for ligand recognition, and intracellular domains that couple to heterotrimeric G proteins. Upon binding CXCL13, CXCR5 activates downstream signaling pathways that promote cytoskeletal rearrangement and directed chemotaxis. Through these mechanisms, CD185 directs B cells and T follicular helper cells into B cell follicles within secondary lymphoid tissues, supporting antibody affinity maturation and adaptive immune responses.

Functionally, CXCR5 is essential for proper germinal center architecture and humoral immunity. Expression of C-X-C Motif Chemokine Receptor 5 defines follicular B cells and T follicular helper cells and is widely used as a marker in immunophenotyping and lymphoid tissue studies. Dysregulated CXCR5 signaling has been implicated in autoimmune diseases, chronic inflammatory conditions, and lymphoid malignancies. In certain B cell lymphomas, altered CD185 expression may influence tumor cell positioning and interactions within the lymphoid microenvironment.

The CXCR5 gene is located on chromosome 11 and is transcriptionally regulated during lymphocyte activation and differentiation. Because C-X-C Motif Chemokine Receptor 5 expression is largely restricted to specific immune cell subsets, detection of this receptor provides insight into lymphocyte trafficking, germinal center dynamics, and immune cell compartmentalization in normal and disease contexts.

The C-X-C Motif Chemokine Receptor 5 antibody (clone CXCR5/13095) is suitable for detecting CXCR5 protein expression in research applications. This CD185 antibody supports investigation of B cell biology, T follicular helper cell function, lymphoid tissue organization, and immune-mediated disease mechanisms.

UniProt: P32302