Recombinant F13A1 antibody (AA 46-181)

Catalog No. ABIN8093094

The Rabbit Monoclonal anti-F13A1 antibody (Clone MSVA-813R) (ABIN8093094) specifically detects F13A1 in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant F13A1 antibody (AA 46-181) (ABIN8093094)

Target: F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This F13A1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-813R

Product Details anti-F13A1 Antibody

Binding Specificity: AA 46-181

Purpose: Factor XIIIa Antibody / Coagulation factor XIII A chain

Immunogen: A recombinant fragment of human Factor XIIIa protein (amino acids 46-181) (exact sequence is proprietary) was used as the immunogen for the F13A1 / Coagulation factor XIII A chain antibody for IHC.

Isotype: IgG, kappa

Application Details

Application Notes: 1. Optimal dilution of the F13A1 Antibody for IHC / Coagulation factor XIII A chain should be determined by the researcher.

2. This F13A1 / Coagulation factor XIII A chain antibody is recombinantly produced by expression in human HEK293 cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: F13A1 / Coagulation factor XIII A chain antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for F13A1

Target: F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))

Alternative Name: Factor XIIIa

Background: F13A1 Antibody for IHC recognizes Coagulation factor XIII A, the catalytic transglutaminase subunit encoded by the F13A1 gene on chromosome 6p25.1. Coagulation factor XIII A, also referred to as Factor XIIIa or Factor XIII A chain, is a cytoplasmic enzyme expressed in platelets, macrophages, dermal dendritic cells, and selected stromal populations. Upon activation by thrombin and calcium, the inactive zymogen is cleaved to generate active Factor XIIIa, which catalyzes covalent cross-linking of fibrin monomers. This reaction stabilizes the fibrin clot and confers resistance to fibrinolysis, representing the final enzymatic step of the coagulation cascade.

Coagulation factor XIII A belongs to the transglutaminase family and contains a catalytic core domain that mediates formation of epsilon-gamma glutamyl-lysine isopeptide bonds between substrate proteins. In plasma, factor XIII circulates as a heterotetramer composed of two catalytic A subunits and two non-catalytic B subunits. Following thrombin-mediated activation, the B subunits dissociate and the A subunits become enzymatically active. Beyond clot stabilization, Factor XIIIa contributes to extracellular matrix remodeling by cross-linking fibronectin, collagen, and other structural proteins, supporting wound healing and tissue repair processes.

In normal tissues, Coagulation factor XIII A expression is prominent in dermal dendrocytes and tissue macrophages. It is also detectable in placenta and bone marrow-derived cells. In fibrohistiocytic lesions, F13A1 Antibody for IHC is frequently applied in research settings to help characterize dermatofibroma, which typically demonstrates strong cytoplasmic positivity in spindle-shaped dermal cells, whereas dermatofibrosarcoma protuberans generally lacks staining. Expression patterns have also been described in inflammatory and reparative conditions involving macrophage activation and stromal remodeling.

Inherited deficiency of Factor XIII results in impaired clot stability and delayed wound healing, underscoring the biologic importance of F13A1 function. Altered expression has been observed in inflammatory and fibrotic disorders, reflecting its broader role in immune cell biology and extracellular matrix stabilization. Clone MSVA-813R is a rabbit monoclonal antibody suitable for detecting Coagulation factor XIII A expression in immunohistochemistry and related research applications, where staining is typically cytoplasmic in dendritic or macrophage-lineage cells.

This antibody is also part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.

UniProt: P00488