Recombinant Cytomegalovirus (CMV) (C-Term) antibody

Catalog No. ABIN8093117

The Mouse Monoclonal anti-Cytomegalovirus antibody is suitable to detect Cytomegalovirus in samples from Human Herpesvirus 5 (HHV-5). It has been validated for WB and IHC.

Quick Overview for Recombinant Cytomegalovirus (CMV) (C-Term) antibody (ABIN8093117)

Target: Cytomegalovirus (CMV)

Antibody Type: Recombinant Antibody

Reactivity: Human Herpesvirus 5 (HHV-5)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: R6

Product Details

Binding Specificity: C-Term

Purpose: Cytomegalovirus Antibody / HCMV pp65

Immunogen: Prokaryotic recombinant protein corresponding to a region at the C-terminal end of the cytomegalovirus pp65 molecule was used as the immunogen for the HCMV pp65/UL83 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the HCMV pp65/UL83 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage Comment: HCMV pp65/UL83 antibody with sodium azide - store at 2 to 8oC

Target Details

Target: Cytomegalovirus (CMV)

Alternative Name: Cytomegalovirus

Target Type: Virus

Background: HCMV pp65 antibody recognizes UL83, also known as Human cytomegalovirus phosphoprotein 65, a major tegument protein of Human cytomegalovirus that plays a central role in viral replication, immune modulation, and virion assembly. HCMV pp65 Antibody targets one of the most abundant structural proteins packaged within the viral tegument layer and is widely used as a marker of active HCMV infection in research settings.

UL83 encodes pp65, a highly immunogenic phosphoprotein that accumulates in the cytoplasm of infected cells during early and late stages of viral replication. Following infection, pp65 is delivered into host cells as part of the incoming virion and rapidly traffics to the nucleus before redistributing to the cytoplasm during viral assembly. As a major tegument protein, pp65 contributes to viral particle maturation and regulates host immune responses, including modulation of interferon signaling and antigen presentation pathways.

The UL83 gene is conserved among clinical isolates of HCMV and is frequently used as a diagnostic and research biomarker due to the protein’s abundance and strong immunogenicity. In infected tissues, pp65 is typically detected within cytoplasmic and perinuclear compartments of infected epithelial, endothelial, and fibroblast populations. Its high expression level makes it a preferred target for immunohistochemical and immunoblot-based detection of HCMV infection in experimental systems.

Because pp65 is a dominant antigen recognized by cytotoxic T lymphocytes, it plays a key role in shaping host adaptive immune responses during primary infection and viral reactivation. Altered pp65 expression patterns have been associated with viral latency, immune evasion strategies, and disease progression in immunocompromised individuals. These characteristics position UL83 as both a structural virion component and an immunomodulatory viral factor.

Clone r6 is a recombinant mouse monoclonal antibody that recognizes HCMV pp65 and supports research applications focused on viral detection, host-virus interaction studies, and characterization of cytomegalovirus infection models.

UniProt: P06725