Recombinant Decorin antibody (AA 212-336)

Catalog No. ABIN8093127

The Rabbit Monoclonal anti-Decorin antibody (Clone MSVA-537R) (ABIN8093127) specifically detects Decorin in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant Decorin antibody (AA 212-336) (ABIN8093127)

Target: Decorin (DCN)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Decorin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-537R

Product Details anti-Decorin Antibody

Binding Specificity: AA 212-336

Purpose: Decorin Antibody / DCN

Immunogen: Recombinant human Decorin protein fragment (around amino acids 212-336) (exact sequence is proprietary) was used as the immunogen for the Decorin antibody for IHC.

Isotype: IgG, kappa

Application Details

Application Notes: 1. Optimal dilution of the DCN/Decorin antibody should be determined by the researcher.

2. This DCN antibody for IHC is recombinantly produced by expression in CHO cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

4. For immunostaining, pre-incubation with chondroitinase-SBC or testicular hyaluronidase may be required to expose the epitope.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: DCN/Decorin antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for Decorin

Target: Decorin (DCN)

Alternative Name: Decorin

Background: Decorin Antibody for IHC detects Decorin (DCN), a small leucine-rich extracellular matrix proteoglycan encoded by the DCN gene and widely distributed in connective tissues. Clone MSVA-537R is a recombinant monoclonal antibody developed for immunohistochemistry and supports visualization of Decorin within stromal extracellular matrix compartments of formalin-fixed tissue sections. This antibody is part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.

Decorin antibody, also referred to as DCN antibody and small leucine-rich proteoglycan Decorin antibody in the literature, recognizes a secreted proteoglycan characterized by tandem leucine-rich repeat domains and a single dermatan sulfate or chondroitin sulfate glycosaminoglycan chain. Decorin binds to fibrillar collagens, particularly type I collagen, where it regulates collagen fibrillogenesis, fiber diameter, and interfibrillar spacing. Through these interactions, Decorin contributes to tissue tensile strength and extracellular matrix stability.

Beyond structural support, Decorin modulates cellular signaling pathways through interactions with growth factors and receptor tyrosine kinases, including transforming growth factor beta and epidermal growth factor receptor. These regulatory effects influence cell proliferation, differentiation, and migration, positioning Decorin as both a matrix scaffold and a signaling modulator within the tissue microenvironment. Decorin expression is prominent in skin, tendon, ligament, placenta, prostate stroma, and other collagen-rich tissues. A Decorin Antibody for IHC typically demonstrates extracellular stromal staining between collagen bundles and surrounding glandular or vascular structures and is commonly used to evaluate extracellular matrix organization, fibrosis, and tumor-associated desmoplastic responses.

Altered Decorin expression has been associated with fibrosis, abnormal wound healing, and tumor progression, where remodeling of collagen architecture and stromal signaling contributes to disease development. Assessment of Decorin distribution may therefore support research into extracellular matrix biology, connective tissue organization, and tumor microenvironment remodeling. This Decorin Antibody for IHC targets Decorin in research applications and is available from NSJ Bioreagents.

This antibody can be compared with our < a href=../tds/decorin-antibody-dcn3521-v5683>Decorin Antibody (clone DCN/3521) for consistent detection of DCN across extracellular matrix and proteoglycan biology studies.

UniProt: P07585

Pathways: Glycosaminoglycan Metabolic Process