Recombinant DKK3 antibody

Catalog No. ABIN8093133

The Rabbit Monoclonal anti-DKK3 antibody (Clone 21D17) (ABIN8093133) specifically detects DKK3 in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Recombinant DKK3 antibody (ABIN8093133)

Target: DKK3

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This DKK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 21D17

Product Details anti-DKK3 Antibody

Purpose: DKK3 Antibody / Dickkopf-related protein 3

Purification: Purified

Immunogen: A synthesized peptide derived from human Dickkopf-related protein 3 was used as the immunogen for the DKK3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the DKK3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the DKK3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for DKK3

Target: DKK3

Background: DKK3 antibody targets Dickkopf-related protein 3, encoded by the DKK3 gene. DKK3 is a secreted glycoprotein belonging to the Dickkopf family, a group of proteins originally identified as modulators of Wnt signaling. Unlike other Dickkopf family members, DKK3 does not function as a classical Wnt antagonist and instead exhibits context-dependent regulatory roles in cell signaling, differentiation, and tissue homeostasis. DKK3 is synthesized intracellularly and secreted into the extracellular space, where it can influence cell-cell communication.

Functionally, Dickkopf-related protein 3 has been implicated in regulation of cell proliferation, apoptosis, and differentiation. DKK3 activity appears to depend on cellular context and tissue type, with reported involvement in modulation of growth factor signaling pathways and stress-responsive cellular programs. Through these mechanisms, DKK3 contributes to maintenance of normal tissue architecture and regulation of cellular responses to environmental cues. A DKK3 antibody supports studies focused on extracellular signaling and regulatory protein function.

DKK3 expression is detected in a variety of tissues, including epithelial and stromal compartments. It is often associated with differentiated cell states and has been reported to localize both intracellularly during synthesis and extracellularly following secretion. Changes in DKK3 expression levels have been linked to alterations in cellular differentiation status and signaling balance.

From a disease-relevance perspective, DKK3 has been widely studied in cancer biology. Reduced or altered DKK3 expression has been reported in multiple tumor types and is frequently associated with tumor progression, invasion, and poor prognosis. DKK3 has therefore been proposed as a tumor suppressor-associated protein and a potential biomarker for disease state and therapeutic response. These associations make DKK3 an important target for research into cancer-related signaling pathways.

At the molecular level, Dickkopf-related protein 3 contains conserved cysteine-rich domains characteristic of the Dickkopf family, which support protein folding and extracellular interactions. Post-translational modifications and secretion dynamics can influence its apparent behavior in biochemical assays. DKK3 antibody reagents support research applications focused on cell signaling, tumor biology, and extracellular regulatory mechanisms, with NSJ Bioreagents providing reagents intended for research use.

UniProt: Q9UBP4