Recombinant Glucagon antibody (AA 1-100)

Catalog No. ABIN8093204

The Rabbit Monoclonal anti-Glucagon antibody is suitable to detect Glucagon in samples from Human. It has been validated for IHC.

Quick Overview for Recombinant Glucagon antibody (AA 1-100) (ABIN8093204)

Target: Glucagon (GCG)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Glucagon antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Grade: Carrier-free

Clone: GCG-13090R

Product Details anti-Glucagon Antibody

Binding Specificity: AA 1-100

Purpose: Glucagon Antibody / GCG (azide and preservative free)

Immunogen: A recombinant fragment (around amino acids 1-100) of human GCG protein (exact sequence is proprietary) was used as the immunogen for the GCG/Glucagon antibody.

Isotype: IgG, kappa

Application Details

Application Notes: 1. Optimal dilution of the GCG/Glucagon antibody should be determined by the researcher.

2. This GCG/Glucagon antibody is recombinantly produced by expression in human HEK293 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: GCG/Glucagon antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for Glucagon

Target: Glucagon (GCG)

Alternative Name: Glucagon

Background: GCG antibody targets Glucagon, a 29 amino acid peptide hormone encoded by the GCG gene and primarily localized to secretory granules within pancreatic alpha cells. Glucagon is synthesized as part of the larger precursor protein Proglucagon, which undergoes tissue-specific proteolytic processing to generate several biologically active peptides. In pancreatic alpha cells, prohormone convertase 2 cleaves Proglucagon to yield mature Glucagon, while alternative processing in intestinal L cells produces peptides such as glucagon-like peptide 1 and glucagon-like peptide 2. Because of this shared precursor, a Glucagon antibody is widely used to study endocrine pancreas biology and proglucagon-derived peptide expression patterns in research settings.

Glucagon plays a central role in systemic glucose homeostasis by counterbalancing insulin action. Upon hypoglycemia, Glucagon is secreted into the circulation and acts primarily on hepatocytes, where it binds the glucagon receptor and stimulates glycogenolysis and gluconeogenesis. This signaling involves activation of adenylate cyclase, increased intracellular cAMP levels, and downstream protein kinase A-mediated phosphorylation of key metabolic enzymes. Through these mechanisms, Glucagon promotes glucose release into the bloodstream and supports energy availability during fasting or metabolic stress. A GCG antibody is therefore valuable for investigating endocrine regulation of metabolism and hormonal control of hepatic glucose output.

Beyond its classical metabolic role, Glucagon has emerged as an important modulator of lipid metabolism, amino acid turnover, and energy expenditure. Studies have shown that Glucagon signaling influences hepatic fatty acid oxidation and ureagenesis, linking amino acid catabolism with glucose regulation. These expanded physiological roles have renewed interest in Glucagon biology, particularly in the context of metabolic disorders. Use of a Glucagon antibody allows researchers to examine alpha cell mass, hormone storage, and secretion dynamics under normal and pathological conditions.

In pancreatic islets, Glucagon-expressing alpha cells are spatially and functionally distinct from insulin-producing beta cells and somatostatin-producing delta cells. Alterations in alpha cell number or Glucagon expression are commonly observed in diabetes mellitus, including type 1 diabetes, type 2 diabetes, and monogenic forms of diabetes affecting islet architecture. A pancreatic Glucagon antibody is frequently applied to characterize islet composition, alpha cell distribution, and endocrine cell plasticity in both human and experimental model tissues.

Glucagon is also relevant in neuroendocrine and gastrointestinal research. Proglucagon-derived peptides share sequence homology, making specificity an important consideration when studying hormone processing pathways. Well-characterized Glucagon antibody reagents enable differentiation between pancreatic Glucagon and related intestinal peptides derived from the same precursor. Clone GCG/13090R is designed to recognize Glucagon and can be applied in studies of hormone expression, endocrine differentiation, and metabolic disease research. NSJ Bioreagents provides this Glucagon antibody as a research tool for investigators examining alpha cell biology, hormone regulation, and metabolic signaling pathways.

Because Glucagon expression is tightly regulated at the transcriptional, translational, and post-translational levels, a GCG antibody is useful for assessing both steady-state hormone levels and adaptive responses to physiological stimuli such as fasting, exercise, or pharmacologic intervention. These applications make Glucagon antibody reagents central to studies of pancreatic endocrine function, diabetes pathogenesis, and metabolic homeostasis.

UniProt: P01275

Pathways: Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Negative Regulation of intrinsic apoptotic Signaling