GPX4 antibody (AA 30-197)

Catalog No. ABIN8093215

This Rabbit Polyclonal antibody specifically detects GPX4 in WB, IP, FACS and IHC (p). It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for GPX4 antibody (AA 30-197) (ABIN8093215)

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPX4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GPX4 Antibody

Binding Specificity: AA 30-197

Purpose: GPX4 Antibody / Glutathione Peroxidase 4

Purification: Antigen affinity

Immunogen: Amino acids A30-F197 of human Glutathione Peroxidase 4 were used as the immunogen for the GPX4 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GPX4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the GPX4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for GPX4

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Alternative Name: GPX4

Background: GPX4 Antibody targets Glutathione Peroxidase 4, a selenium-dependent antioxidant enzyme encoded by the GPX4 gene that plays a critical role in protecting cells from oxidative damage. Glutathione Peroxidase 4 belongs to the glutathione peroxidase family and is unique among its members due to its ability to directly reduce lipid hydroperoxides within biological membranes. Through this activity, GPX4 contributes to maintenance of membrane integrity and cellular redox balance.

Functionally, Glutathione Peroxidase 4 catalyzes the reduction of phospholipid hydroperoxides and cholesterol hydroperoxides using glutathione as a reducing agent. This activity distinguishes GPX4 from other glutathione peroxidases that primarily act on soluble peroxide substrates. By limiting lipid peroxidation, GPX4 prevents oxidative damage to cellular membranes and associated macromolecules. A GPX4 Antibody enables investigation of antioxidant defense mechanisms and lipid redox regulation in research studies focused on cellular stress responses.

GPX4 expression is observed in many tissues, reflecting its importance in fundamental cellular protection mechanisms. At the subcellular level, Glutathione Peroxidase 4 is found in multiple compartments, including the cytoplasm, nucleus, and mitochondria, depending on isoform and cellular context. This broad distribution allows GPX4 to protect diverse cellular structures from oxidative injury. Changes in GPX4 expression or localization may indicate altered redox state, metabolic stress, or activation of cell death pathways.

At the molecular level, GPX4 contains a conserved catalytic site that incorporates the amino acid selenocysteine, which is essential for its peroxidase activity. This structural feature confers high reactivity toward lipid hydroperoxides and enables efficient detoxification of oxidized lipids. Regulation of GPX4 expression and activity occurs at transcriptional and post-translational levels and is influenced by cellular selenium availability and redox conditions.

From a biological and disease relevance perspective, GPX4 has been extensively studied in the context of ferroptosis, a regulated form of cell death driven by lipid peroxidation. Loss or inhibition of GPX4 activity leads to accumulation of lipid peroxides and subsequent membrane damage, triggering ferroptotic cell death. As a result, GPX4 is a central focus of research into oxidative stress, neurodegeneration, cancer biology, and metabolic disease, where redox imbalance plays a pathogenic role.

GPX4 Antibody reagents are valuable tools for studying antioxidant enzyme function, lipid peroxidation control, and redox-dependent cell death pathways. These antibodies support research into oxidative stress responses, ferroptosis regulation, and disease-associated alterations in cellular antioxidant defenses. NSJ Bioreagents provides GPX4 Antibody products intended for research use.

UniProt: P36969