Recombinant HTATSF1 antibody

Catalog No. ABIN8093236

This Rabbit Monoclonal antibody specifically detects HTATSF1 in WB, IHC (p) and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Recombinant HTATSF1 antibody (ABIN8093236)

Target: HTATSF1 (HIV-1 Tat Specific Factor 1 (HTATSF1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HTATSF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: 25H56

Product Details anti-HTATSF1 Antibody

Purpose: HTATSF1 Antibody / HIV Tat-specific factor 1

Purification: Purified

Immunogen: A synthesized peptide derived from human HIV Tat-specific factor 1 protein was used as the immunogen for the HIV Tat-specific factor 1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the HIV Tat-specific factor 1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the HIV Tat-specific factor 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for HTATSF1

Target: HTATSF1 (HIV-1 Tat Specific Factor 1 (HTATSF1))

Alternative Name: HTATSF1

Background: HIV Tat-specific factor 1 antibody targets HIV Tat-specific factor 1, encoded by the HTATSF1 gene on chromosome 17q21.31. HIV Tat-specific factor 1 is a nuclear regulatory protein that functions at the intersection of transcription elongation and RNA processing. Although first identified for its role in supporting HIV-1 Tat-mediated transcription, HIV Tat-specific factor 1 is now recognized as a broader host factor that contributes to efficient gene expression in mammalian cells.

Functionally, HIV Tat-specific factor 1 participates in regulation of RNA polymerase II elongation rather than transcription initiation. It associates with elongating transcription complexes and supports productive transcript synthesis across actively transcribed genes. In parallel, HIV Tat-specific factor 1 facilitates coordination between transcriptional elongation and RNA processing events, allowing efficient coupling of nascent RNA synthesis with downstream maturation steps. A HIV Tat-specific factor 1 antibody supports studies focused on transcriptional elongation dynamics and RNA metabolism.

HIV Tat-specific factor 1 localizes predominantly to the nucleus, where it interacts with components of the transcription and splicing machinery. The protein contains arginine-serine-rich regions and additional interaction motifs characteristic of RNA regulatory factors, enabling binding to RNA and protein partners within elongation and spliceosomal complexes. Alternative splicing of HTATSF1 transcripts may generate isoforms that modulate these regulatory activities in a context-dependent manner.

From a biological and disease-relevance perspective, accurate regulation of transcription elongation and RNA processing is essential for cellular homeostasis. Dysregulated activity of HIV Tat-specific factor 1 has been investigated in cancer, where aberrant transcriptional control contributes to altered gene expression programs. In viral infection models, HIV Tat-specific factor 1 remains relevant due to its ability to enhance Tat-dependent transcription, illustrating how host transcription factors can be co-opted to support viral replication.

At the molecular level, HIV Tat-specific factor 1 functions as a regulatory protein rather than an enzyme, acting through dynamic protein-protein and protein-RNA interactions. Post-translational regulation and complex assembly state can influence its functional behavior and apparent migration in biochemical assays without altering the primary amino acid sequence. HIV Tat-specific factor 1 antibody reagents support research applications focused on transcription elongation, RNA processing, and host-virus interactions, with NSJ Bioreagents providing reagents intended for research use.

UniProt: O43719