Recombinant L1CAM antibody

Catalog No. ABIN8093292

The Rabbit Monoclonal anti-L1CAM antibody (Clone AAFD-12) (ABIN8093292) specifically detects L1CAM in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Recombinant L1CAM antibody (ABIN8093292)

Target: L1CAM (L1 Cell Adhesion Molecule (L1CAM))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This L1CAM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: AAFD-12

Product Details anti-L1CAM Antibody

Purpose: L1CAM Antibody / NCAM-L1 / CD171

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human Neural cell adhesion molecule L1 protein was used as the immunogen for the NCAM-L1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NCAM-L1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the NCAM-L1 antibody at -20oC.

Target Details for L1CAM

Target: L1CAM (L1 Cell Adhesion Molecule (L1CAM))

Alternative Name: L1CAM

Background: NCAM-L1 antibody targets Neural cell adhesion molecule L1 (NCAM-L1), also known as L1CAM and CD171, a transmembrane glycoprotein belonging to the immunoglobulin superfamily of cell adhesion molecules. NCAM-L1 is predominantly localized to the plasma membrane, where it mediates calcium-independent cell-cell interactions that are essential for nervous system development and tissue organization. The extracellular region of NCAM-L1 contains multiple immunoglobulin-like and fibronectin type III domains that enable both homophilic binding between adjacent cells and heterophilic interactions with extracellular matrix components and receptors. These structural features position NCAM-L1 as a key organizer of cell surface architecture in developing and mature tissues.

Functionally, Neural cell adhesion molecule L1 plays a central role in neuronal migration, axon guidance, axon fasciculation, and synaptic organization. Through its adhesive properties and associated signaling functions, NCAM-L1 coordinates directional cell movement and neurite outgrowth during neural circuit formation. The cytoplasmic domain of NCAM-L1 interacts with adaptor proteins and cytoskeletal elements, linking extracellular adhesion events to intracellular signaling pathways that regulate cell shape, motility, and polarity. An NCAM-L1 antibody supports studies examining how adhesion molecules integrate structural and signaling roles during development.

Expression of NCAM-L1 is highest in the developing nervous system, where it is detected in neurons and certain glial populations, but it is also present in selected non-neural tissues where cell migration and dynamic tissue remodeling occur. NCAM-L1 expression is tightly regulated in both spatial and temporal patterns, reflecting its importance in guiding cells through complex microenvironments. Analysis of NCAM-L1 localization and abundance provides insight into mechanisms that govern tissue patterning, connectivity, and structural integrity.

From a biological and disease-relevance perspective, NCAM-L1 has been extensively studied in neurodevelopmental disorders, where altered expression or function can disrupt neuronal connectivity and migration. NCAM-L1 is also investigated in cancer biology, as its expression has been associated with increased cell motility, invasiveness, and metastatic potential in certain tumor types. These observations highlight the broader relevance of NCAM-L1 beyond normal development and underscore its value as a marker of cellular plasticity and migratory behavior.

At the molecular level, NCAM-L1 is encoded by the L1CAM gene and produces a heavily glycosylated protein that typically migrates at approximately 200 to 220 kDa on SDS-PAGE, depending on glycosylation state and proteolytic processing. Multiple molecular forms may be detected due to post-translational modification and cleavage events. Regulation of NCAM-L1 function depends on expression level, membrane distribution, and interaction with binding partners. An NCAM-L1 antibody supports research applications focused on cell adhesion, neural development, and migration-related signaling pathways, with NSJ Bioreagents providing reagents intended for research use.

UniProt: P32004

Pathways: Synaptic Membrane