LI Cadherin antibody (AA 242-418)

Catalog No. ABIN8093299

This Mouse Monoclonal antibody specifically detects LI Cadherin in IHC. It exhibits reactivity toward Human.

Quick Overview for LI Cadherin antibody (AA 242-418) (ABIN8093299)

Target: LI Cadherin

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LI Cadherin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-517M

Product Details anti-LI Cadherin Antibody

Binding Specificity: AA 242-418

Purpose: CDH17 Antibody / Liver intestine cadherin

Immunogen: A recombinant fragment (around amino acids 242-418) of human Cadherin 17 protein (CDH17) (exact sequence is proprietary) was used as the immunogen for the CDH17 antibody for IHC.

Isotype: IgG2b, kappa

Application Details

Application Notes: 1. Optimal dilution of the CDH17 antibody for IHC should be determined by the researcher.

2. This CDH17 antibody for IHC is recombinantly produced by expression in CHO cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: CDH17/Liver intestine cadherin antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for LI Cadherin

Target: LI Cadherin

Alternative Name: CDH17

Background: CDH17 antibody recognizes Cadherin 17, a calcium-dependent cell adhesion molecule encoded by the CDH17 gene located on chromosome 8q22.1. CDH17 Antibody for IHC is optimized for detecting this intestinal-type cadherin in formalin-fixed, paraffin-embedded tissue specimens. Cadherin 17 is widely known as Liver-intestine cadherin and LI-cadherin, and it differs structurally from classical cadherins because it lacks the conserved cytoplasmic catenin-binding domain. The protein localizes primarily to the plasma membrane of epithelial cells, where it supports calcium-dependent adhesion and maintenance of epithelial architecture in gastrointestinal tissues.

Liver intestine cadherin antibody, also referred to as Cadherin 17 antibody and CDH17 antibody in the literature, targets a member of the cadherin superfamily characterized by seven extracellular cadherin repeats and a short cytoplasmic tail. Unlike E-cadherin, Cadherin 17 mediates adhesion independently of beta-catenin interaction. It is strongly expressed in small intestine and colon epithelium and is also detected in pancreatic ducts and certain gastric epithelial cells. In tissue sections, staining is typically membranous and highlights glandular epithelial structures with preserved polarity.

Aberrant CDH17 expression has been reported in colorectal adenocarcinoma, gastric carcinoma, pancreatic adenocarcinoma, and other gastrointestinal malignancies. In tumor specimens, Cadherin 17 frequently demonstrates strong membranous staining in well-differentiated epithelial tumor cells, reflecting maintenance of intestinal lineage features. Altered or reduced expression patterns may correlate with tumor progression or dedifferentiation in certain settings. CDH17 antibody is therefore widely used in research evaluating gastrointestinal tumor differentiation and epithelial lineage identity.

Beyond its adhesive function, Cadherin 17 has been implicated in signaling pathways influencing cell proliferation and migration. Experimental evidence suggests that CDH17 may contribute to tumor growth and survival in colorectal cancer models. Its consistent expression in intestinal-type epithelium supports its value as a marker of gastrointestinal epithelial differentiation.

This mouse monoclonal antibody clone MSVA-517M targets Cadherin 17 for tissue-based research applications. By enabling visualization of CDH17 expression and membranous localization in formalin-fixed specimens, this Liver intestine cadherin antibody supports investigations into gastrointestinal epithelial biology at NSJ Bioreagents.

This antibody is also part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.

UniProt: Q12864