Recombinant NME2 antibody (full length)

Catalog No. ABIN8093368

This Rabbit Monoclonal antibody specifically detects NME2 in WB. It exhibits reactivity toward Human.

Quick Overview for Recombinant NME2 antibody (full length) (ABIN8093368)

Target: NME2 (Nucleoside diphosphate kinase B (NME2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NME2 antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Clone: NME2-12953R

Product Details anti-NME2 Antibody

Binding Specificity: full length

Purpose: NME2 Antibody / Nucleoside diphosphate kinase B (azide and preservative free)

Immunogen: Recombinant full-length human NME2 protein was used as the immunogen for the nm23-H2/NME2 antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the nm23-H2/NME2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: nm23-H2/NME2 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for NME2

Target: NME2 (Nucleoside diphosphate kinase B (NME2))

Alternative Name: NME2

Background: Nm23-H2 antibody recognizes Nucleoside diphosphate kinase B, a multifunctional enzyme encoded by the human NME2 gene that plays a central role in nucleotide metabolism and cellular signaling. nm23-H2 antibody detects a ubiquitously expressed cytoplasmic protein that catalyzes the transfer of terminal phosphates between nucleoside triphosphates and diphosphates, thereby maintaining balanced intracellular pools of ATP, GTP, CTP, and UTP required for DNA replication, RNA transcription, and energy-dependent signaling pathways. The protein is primarily localized in the cytoplasm but may also be detected in the nucleus depending on cellular context.

nm23-H2 antibody, also referred to as NME2 antibody and Nucleoside diphosphate kinase B antibody in the literature, targets a member of the NME gene family. The nm23-H2 protein forms a functional hexamer and contains a conserved histidine residue within its catalytic domain that undergoes transient phosphorylation during phosphotransfer reactions. Through regulation of GTP availability, nm23-H2 contributes to signaling pathways involving small GTP-binding proteins that control proliferation, cytoskeletal organization, and vesicle trafficking.

The NME2 gene is located on chromosome 17q21.3 and is closely related to NME1, another nm23 family member historically associated with metastasis suppression. Beyond its enzymatic activity, nm23-H2 has been implicated in transcriptional regulation and protein-protein interactions that influence gene expression and cellular differentiation. These additional functions expand its biological relevance beyond nucleotide homeostasis.

Altered expression of nm23 family proteins has been reported in multiple tumor types, where expression levels may correlate with tumor progression or metastatic behavior depending on cellular and tissue context. Because nm23-H2 influences signaling networks linked to growth control and motility, it has been studied extensively in cancer biology and developmental systems.

Expression of nm23-H2 is widespread in normal tissues, consistent with its fundamental metabolic role. Its predominantly cytoplasmic distribution, with occasional nuclear staining, makes nm23-H2 antibody useful for investigating nucleotide metabolism and regulatory signaling pathways in both normal and transformed cells.

Clone NME2/12953R is a recombinant monoclonal antibody developed to detect nm23-H2 protein in research applications. An antibody to nm23-H2 is suitable for studying NME2 expression and for examining cellular metabolism and signal transduction mechanisms in relevant experimental systems.

UniProt: P22392