PDLIM5 antibody (Middle Region)

Catalog No. ABIN8093395

This Rabbit Polyclonal antibody specifically detects PDLIM5 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PDLIM5 antibody (Middle Region) (ABIN8093395)

Target: PDLIM5 (PDZ and LIM Domain 5 (PDLIM5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDLIM5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PDLIM5 Antibody

Binding Specificity: Middle Region

Purpose: PDLIM5 Antibody / PDZ and LIM domain protein 5

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PDZ and LIM domain protein 5 protein was used as the immunogen for the PDLIM5 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PDLIM5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PDLIM5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PDLIM5

Target: PDLIM5 (PDZ and LIM Domain 5 (PDLIM5))

Alternative Name: PDLIM5

Background: PDLIM5 antibody targets PDZ and LIM domain protein 5, encoded by the PDLIM5 gene. PDLIM5 is a cytoplasmic and cytoskeletal-associated protein that belongs to the PDZ-LIM family, characterized by an N-terminal PDZ domain and multiple C-terminal LIM domains. These modular interaction motifs enable PDLIM5 to function as a scaffold protein that links signaling molecules to the actin cytoskeleton, supporting spatial organization of signaling complexes within the cell.

PDZ and LIM domain protein 5 is widely expressed, with notable roles in muscle cells, neurons, and other mechanically active tissues. Through its PDZ domain, PDLIM5 associates with actin-binding proteins and cytoskeletal elements, while its LIM domains mediate interactions with kinases and signaling adaptors. This positioning allows PDLIM5 to coordinate structural integrity with intracellular signaling pathways, particularly those involved in stress responses and cellular remodeling.

Functionally, PDLIM5 participates in pathways regulating cell shape, adhesion, and signal transduction. It has been linked to modulation of protein kinase signaling cascades, including pathways that influence cell survival and differentiation. In excitable tissues such as cardiac and neural systems, PDLIM5 contributes to organization of signaling hubs that respond to mechanical and biochemical cues. A PDLIM5 antibody is therefore useful for examining how cytoskeletal scaffolds integrate signaling events at specific subcellular locations.

PDLIM5 has attracted research interest due to its reported involvement in neurological and cardiovascular biology. Altered expression or regulation of PDLIM5 has been explored in the context of psychiatric disorders, cardiomyopathy, and stress-related cellular responses. While mechanistic details continue to be refined, these associations highlight the importance of PDZ-LIM scaffold proteins in maintaining normal cellular function and signaling balance.

At the molecular level, PDZ and LIM domain protein 5 exists in multiple isoforms generated through alternative splicing, which may differ in interaction capacity and subcellular distribution. Post-translational modifications and binding-partner availability can influence its apparent behavior in biochemical assays without altering the underlying amino acid sequence. PDLIM5 antibody reagents support research applications focused on cytoskeletal signaling, scaffold protein biology, and disease-associated alterations in intracellular organization, with NSJ Bioreagents providing antibodies intended for research use.

UniProt: Q96HC4