Plakophilin 3 antibody (AA 172-397)

Catalog No. ABIN8093411

The Rabbit Polyclonal anti-Plakophilin 3 antibody is suitable to detect Plakophilin 3 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and IF.

Quick Overview for Plakophilin 3 antibody (AA 172-397) (ABIN8093411)

Target: Plakophilin 3 (PKP3)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Plakophilin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Plakophilin 3 Antibody

Binding Specificity: AA 172-397

Purpose: PKP3 Antibody / Plakophilin 3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human Plakophilin 3 recombinant protein (amino acids S172-L397) was used as the immunogen for the PKP3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PKP3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PKP3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Plakophilin 3

Target: Plakophilin 3 (PKP3)

Alternative Name: PKP3

Background: PKP3 antibody targets Plakophilin 3, encoded by the PKP3 gene. Plakophilin 3 is a member of the armadillo repeat protein family and functions as a structural component of desmosomes, specialized cell-cell junctions that provide strong intercellular adhesion in epithelial tissues. PKP3 localizes predominantly to desmosomal plaques at the plasma membrane, where it interacts with desmosomal cadherins, desmoplakin, and intermediate filaments to reinforce tissue cohesion and mechanical stability. In addition to its junctional localization, PKP3 can also be detected in the cytoplasm, reflecting dynamic regulation of desmosome assembly and turnover.

Functionally, Plakophilin 3 contributes to the formation, stabilization, and regulation of desmosomes, supporting epithelial integrity and resistance to mechanical stress. By linking cadherin-based adhesion complexes to the intermediate filament network, PKP3 helps maintain tissue architecture and barrier function. Beyond its structural role, PKP3 has been implicated in signaling processes that influence cell proliferation, migration, and differentiation, suggesting that desmosomal components can also participate in broader cellular regulatory networks. A PKP3 antibody supports studies focused on cell adhesion mechanisms and epithelial junction biology.

PKP3 expression is most prominent in stratified and simple epithelial tissues, including skin, gastrointestinal epithelium, and other mucosal surfaces. Its expression pattern overlaps with other plakophilins but shows tissue-specific differences that reflect specialized roles in distinct epithelial contexts. PKP3 expression is regulated during development and epithelial differentiation, highlighting its importance in maintaining mature epithelial structure and function. Changes in PKP3 localization or abundance can accompany epithelial remodeling and stress responses.

From a disease-relevance perspective, altered PKP3 expression has been investigated in cancer biology, particularly in epithelial-derived tumors. Dysregulation of desmosomal components, including Plakophilin 3, can contribute to reduced cell-cell adhesion, increased cellular motility, and invasive behavior during tumor progression. PKP3 has also been studied in inflammatory and skin-related disorders, where compromised desmosomal integrity can impair barrier function and tissue resilience. These associations make PKP3 a relevant marker in studies of epithelial pathology and junctional dysregulation.

At the molecular level, Plakophilin 3 contains multiple armadillo repeat domains that facilitate protein-protein interactions within desmosomal complexes. Isoform diversity and post-translational modifications can influence its interaction partners, subcellular distribution, and electrophoretic behavior on SDS-PAGE without necessarily indicating changes in primary protein structure. A PKP3 antibody supports research applications focused on desmosome organization, epithelial biology, and disease-associated alterations in cell adhesion, with NSJ Bioreagents providing reagents intended for research use.

UniProt: Q9Y446