Recombinant PVRL4 antibody (Extracellular)

Catalog No. ABIN8093419

This Rabbit Monoclonal antibody specifically detects PVRL4 in IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant PVRL4 antibody (Extracellular) (ABIN8093419)

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PVRL4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Carrier-free

Clone: NECTIN4-13438R

Product Details anti-PVRL4 Antibody

Binding Specificity: AA 266-280, Extracellular

Purpose: NECTIN4 Antibody / Poliovirus receptor related 4 (azide and preservative free)

Immunogen: A recombinant fragment (around amino acids 266-280) of human NECTIN4 protein corresponding to the extracellular domain (exact sequence is proprietary) was used as the immunogen for the Poliovirus receptor related 4/NECTIN4 antibody.

Isotype: IgG, kappa

Application Details

Application Notes: 1. Optimal dilution of the Poliovirus receptor related 4/NECTIN4 antibody should be determined by the researcher.

2. This Poliovirus receptor related 4/NECTIN4 antibody is recombinantly produced by expression in CHO cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Poliovirus receptor related 4/NECTIN4 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for PVRL4

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Alternative Name: NECTIN4

Background: Poliovirus receptor related 4 antibody, also known as NECTIN4 antibody, recognizes Poliovirus receptor related 4, a type I transmembrane cell adhesion molecule encoded by the NECTIN4 gene. Commonly referred to as Nectin-4 and also designated PVRL4, this protein is a member of the nectin family within the immunoglobulin superfamily. NECTIN4 localizes predominantly to the cell membrane at adherens junctions, where it participates in Ca2+-independent cell-cell adhesion and contributes to maintenance of epithelial tissue architecture. Poliovirus receptor related 4 antibody is widely used in studies of epithelial biology and tumor-associated membrane protein expression.

NECTIN4 contains three extracellular immunoglobulin-like domains, a single transmembrane segment, and a cytoplasmic tail that interacts with intracellular adaptor proteins involved in junctional organization and cytoskeletal linkage. Through homophilic and heterophilic interactions with other nectin family members, Poliovirus receptor related 4 regulates cell polarity, adhesion strength, and tissue integrity. In normal tissues, expression is relatively restricted, with detectable levels in skin, placenta, and certain epithelial compartments. During development, nectin family proteins contribute to tissue morphogenesis and coordinated cell positioning.

Aberrant overexpression of Nectin-4 has been documented in multiple malignancies, including urothelial carcinoma, breast cancer, lung cancer, and ovarian cancer. Elevated NECTIN4 expression has been associated with enhanced proliferation and tumor progression, and the protein has been explored as a surface biomarker in oncology research. Immunostaining typically demonstrates membranous localization in positive tumor cells, consistent with its function as a cell surface adhesion molecule. Clone NECTIN4/13438R is a recombinant rabbit monoclonal antibody generated through defined sequence expression, supporting consistency between production lots. This Poliovirus receptor related 4 antibody enables investigation of NECTIN4 expression in research applications focused on epithelial adhesion, tumor biology, and membrane-associated signaling pathways.

UniProt: Q96NY8

Pathways: Cell-Cell Junction Organization