Recombinant SATB2 antibody (AA 200-300)

Catalog No. ABIN8093456

The Rabbit Monoclonal anti-SATB2 antibody is suitable to detect SATB2 in samples from Human. It has been validated for IHC.

Quick Overview for Recombinant SATB2 antibody (AA 200-300) (ABIN8093456)

Target: SATB2 (SATB Homeobox 2 (SATB2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SATB2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-702R

Product Details anti-SATB2 Antibody

Binding Specificity: AA 200-300

Purpose: SATB2 Antibody / Transcription factor SATB2

Immunogen: Synthetic peptide corresponding to residues within aa200-300 corresponding to human SATB2 was used as the immunogen for the SATB2/Transcription factor SATB2 antibody for IHC.

Isotype: IgG, kappa

Application Details

Application Notes: 1. Optimal dilution of the SATB2/Transcription factor SATB2 antibody for IHC should be determined by the researcher.

2. This SATB2/Transcription factor SATB2 antibody is recombinantly produced by expression in CHO cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: SATB2/Transcription factor SATB2 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for SATB2

Target: SATB2 (SATB Homeobox 2 (SATB2))

Alternative Name: SATB2

Background: SATB2 Antibody for IHC recognizes Transcription factor SATB2, also known as Special AT-rich sequence-binding protein 2, encoded by the SATB2 gene on chromosome 2q33.1. SATB2 is a nuclear matrix-associated transcription factor that functions as a chromatin organizer, binding AT-rich DNA elements and coordinating higher-order chromatin architecture with gene expression. The protein contains two CUT domains and a homeodomain that enable sequence-specific DNA binding and recruitment of transcriptional regulatory complexes. SATB2 localizes predominantly to the nucleus, where it exhibits a granular or reticular staining pattern reflecting its role in chromatin remodeling and transcriptional control.

SATB2 plays a central role in embryonic development, particularly in cortical neuron differentiation, craniofacial patterning, and osteoblast lineage commitment. In the developing cerebral cortex, SATB2 regulates projection neuron identity and connectivity by controlling transcriptional networks essential for neuronal specification. In skeletal tissues, it supports osteogenic differentiation and bone formation by modulating genes involved in extracellular matrix production and mineralization. In adult tissues, SATB2 expression is most prominent in glandular epithelial cells of the lower gastrointestinal tract, especially colorectal mucosa, where it contributes to maintenance of regional epithelial identity and differentiation.

In research and pathology-focused investigations, SATB2 is widely studied as a nuclear marker of colorectal epithelial origin. Strong nuclear expression is frequently observed in colorectal adenocarcinomas, supporting its use in studies evaluating tumor differentiation and lineage. SATB2 expression has also been reported in osteogenic tumors and select lower gastrointestinal neoplasms, reflecting its developmental and tissue-specific functions.

Clone MSVA-702R is a rabbit monoclonal antibody designed for immunohistochemical detection of SATB2 in formalin-fixed, paraffin-embedded tissues. Epitope-specific recognition supports clear nuclear staining with minimal background in optimized conditions. SATB2 Antibody for IHC (MSVA-702R) is suitable for detecting nuclear SATB2 expression in research applications focused on colorectal biology, neuronal development, skeletal differentiation, and chromatin organization.

This antibody is also part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.

Researchers investigating colorectal tumor markers, epithelial lineage determination, and chromatin-associated transcriptional regulation may also be interested in our < a href=../tds/recombinant-satb2-antibody-satb24374r-v8660>SATB2 Antibody / Colorectal and Lineage Marker page featuring validated immunohistochemistry and western blot applications for colorectal pathology research.

UniProt: Q9UPW6