Recombinant SPARC antibody (C-Term)

Catalog No. ABIN8093458

This Mouse Monoclonal antibody specifically detects SPARC in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant SPARC antibody (C-Term) (ABIN8093458)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPARC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Clone: ROSTN-9835

Product Details anti-SPARC Antibody

Binding Specificity: C-Term

Purpose: SPARC Antibody / Secreted protein acidic and cysteine rich (azide and preservative free)

Immunogen: A recombinant fragment corresponding to the C-terminus of human SPARC protein (exact sequence is proprietary) was used as the immunogen for the BM-40/SPARC antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the BM-40/SPARC antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: BM-40/SPARC antibody with sodium azide store at 2 to 8oC, antibody without sodium azide store at -20 to -80oC.

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: SPARC

Background: BM-40 antibody, also known as SPARC antibody, recognizes Secreted protein acidic and rich in cysteine, a secreted matricellular glycoprotein that regulates extracellular matrix organization and cell-matrix communication. The human SPARC gene is located on chromosome 5q33.1 and encodes a protein widely referred to in the literature as Osteonectin and Basement membrane protein 40. SPARC is secreted into the extracellular space and localizes to basement membranes and stromal compartments, where it modulates interactions between cells and the surrounding matrix rather than functioning as a structural scaffold.

Secreted protein acidic and rich in cysteine plays a key role in collagen binding, matrix assembly, and regulation of cell adhesion and migration. It is highly expressed in connective tissues, bone, activated fibroblasts, and endothelial cells. In skeletal biology, Osteonectin is abundant in osteoblasts and bone matrix, contributing to collagen fibrillogenesis and mineralization processes. A BM-40 antibody is frequently used in studies investigating extracellular matrix remodeling, tumor microenvironment dynamics, angiogenesis, and fibrotic disease due to SPARC's involvement in stromal activation and matrix deposition.

Structurally, SPARC contains an acidic N-terminal domain, a follistatin-like domain, and a C-terminal extracellular calcium-binding domain with EF-hand motifs. These domains support calcium binding and mediate interactions with collagens, albumin, and growth factors. Through these molecular interactions, Secreted protein acidic and rich in cysteine influences signaling pathways such as TGF-beta signaling and integrin-mediated adhesion cascades, impacting tissue remodeling and vascular responses.

In cancer research, elevated SPARC expression is commonly observed in desmoplastic stroma associated with breast, pancreatic, colorectal, and ovarian carcinomas. Expression is often detected in tumor-associated fibroblasts and matrix-rich regions, where SPARC contributes to extracellular matrix reorganization. Depending on tumor context, SPARC may be linked to invasion, stromal remodeling, or modulation of growth factor signaling. Beyond oncology, SPARC is upregulated during wound healing and in fibrotic disorders affecting liver, lung, and kidney, reflecting its broader role in tissue repair and matrix turnover. During embryogenesis, SPARC expression is detected in tissues undergoing active morphogenesis and vascular development.

Clone rOSTN/9835 is a recombinant monoclonal antibody developed to recognize SPARC in research applications. A BM-40 antibody supports investigation of extracellular matrix biology, connective tissue remodeling, and stromal cell function. This antibody targets SPARC in research settings and is suitable for studies focused on fibrosis, angiogenesis, skeletal biology, and tumor-associated stromal responses.

UniProt: P09486

Pathways: Autophagy