Recombinant Selectin E/CD62e antibody

Catalog No. ABIN8093463

The Mouse Monoclonal anti-Selectin E/CD62e antibody (Clone r16G4) (ABIN8093463) specifically detects Selectin E/CD62e in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant Selectin E/CD62e antibody (ABIN8093463)

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Selectin E/CD62e antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Grade: Carrier-free

Clone: R16G4

Product Details anti-Selectin E/CD62e Antibody

Purpose: SELE Antibody / E-selectin (azide and preservative free)

Immunogen: Prokaryotic recombinant fusion protein corresponding to the cysteine-rich complement control protein domains of the CD62E molecule was used as the immunogen for the SELE/E-selectin antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: 1. Optimal dilution of the SELE/E-selectin antibody should be determined by the researcher.

2. This SELE/E-selectin antibody is recombinantly produced by expression in CHO cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: SELE/E-selectin antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for Selectin E/CD62e

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Alternative Name: SELE

Background: SELE antibody, also known as E-selectin antibody, recognizes E-selectin, a type I transmembrane adhesion molecule encoded by the SELE gene and commonly referred to as CD62E and endothelial-leukocyte adhesion molecule 1. E-selectin is primarily localized to the plasma membrane of activated endothelial cells and is a member of the selectin family of calcium-dependent lectins. Under basal conditions, SELE expression is low or absent, however, it is rapidly induced in endothelial cells in response to inflammatory cytokines such as interleukin-1 and tumor necrosis factor alpha, linking vascular activation to immune cell recruitment.

SELE antibody detects a glycoprotein composed of an N-terminal C-type lectin domain, an epidermal growth factor-like domain, multiple short consensus repeat units, a single transmembrane region, and a short cytoplasmic tail. The lectin domain mediates binding to sialylated carbohydrate ligands, including sialyl Lewis x structures on neutrophils, monocytes, and subsets of lymphocytes. Through these interactions, E-selectin supports leukocyte tethering and rolling along the vascular endothelium under shear flow conditions, representing a critical early step in extravasation during inflammation.

Functionally, E-selectin plays a central role in acute and chronic inflammatory responses by regulating leukocyte trafficking into tissues. Because its expression is tightly controlled and transient following cytokine stimulation, SELE is widely used as a marker of endothelial activation in experimental models of vascular inflammation. Elevated E-selectin levels have been associated with atherosclerosis, cardiovascular disease, autoimmune disorders, and other inflammatory conditions. In oncology research, endothelial E-selectin can contribute to adhesion of circulating tumor cells, implicating SELE in metastatic dissemination and tumor-vascular interactions.

The SELE gene is located on chromosome 1 and is transcriptionally regulated by inflammatory signaling pathways, including those downstream of nuclear factor kappa B activation. The endothelial-restricted and inducible nature of E-selectin expression makes detection of this protein valuable for studying cytokine signaling, vascular biology, and immune-endothelial interactions.

This recombinant monoclonal SELE antibody (clone r16G4) targets E-selectin protein in research applications. SELE antibody supports investigation of endothelial activation, leukocyte recruitment mechanisms, and disease-associated inflammatory signaling.

UniProt: P16581

Pathways: Thromboxane A2 Receptor Signaling