UCHL3 antibody (C-Term)

Catalog No. ABIN8093545

This Rabbit Polyclonal antibody specifically detects UCHL3 in WB, ELISA, IP and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for UCHL3 antibody (C-Term) (ABIN8093545)

Target: UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCHL3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-UCHL3 Antibody

Binding Specificity: AA 1-223, C-Term

Purpose: UCHL3 Antibody / Ubiquitin C-terminal hydrolase L3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human UCHL3 recombinant protein (amino acids M1-N223) was used as the immunogen for the Ubiquitin C-terminal hydrolase L3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Ubiquitin C-terminal hydrolase L3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Ubiquitin C-terminal hydrolase L3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for UCHL3

Target: UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

Alternative Name: UCHL3

Background: Ubiquitin C-terminal hydrolase L3 antibody targets Ubiquitin C-terminal hydrolase L3, encoded by the UCHL3 gene. Ubiquitin C-terminal hydrolase L3 is an intracellular deubiquitinating enzyme that catalyzes the cleavage of ubiquitin from the C-terminus of ubiquitin-conjugated substrates. As a member of the ubiquitin C-terminal hydrolase family, it plays a fundamental role in regulating ubiquitin-dependent protein modification and turnover.

Unlike substrate-specific ubiquitin ligases, Ubiquitin C-terminal hydrolase L3 functions as a broad regulator of ubiquitin recycling. By regenerating free ubiquitin from conjugated or precursor forms, it sustains the availability of ubiquitin required for continuous proteasomal degradation and signaling regulation. A Ubiquitin C-terminal hydrolase L3 antibody supports studies examining deubiquitination dynamics and maintenance of ubiquitin homeostasis within the cell.

Ubiquitin C-terminal hydrolase L3 is primarily localized to the cytoplasm, where it interfaces with ubiquitinated proteins involved in signaling cascades, metabolic control, and stress response pathways. Its widespread expression across cell types reflects a housekeeping role that supports core cellular functions rather than tissue-restricted specialization. Modulation of UCHL3 activity can therefore influence multiple pathways simultaneously through indirect effects on ubiquitin availability.

From a biological and disease-related perspective, altered regulation of Ubiquitin C-terminal hydrolase L3 has been investigated in cancer and models of cellular stress, where imbalance between ubiquitination and deubiquitination contributes to abnormal protein stabilization or degradation. Changes in UCHL3 expression or activity may impact cell cycle progression, survival signaling, and proteotoxic stress tolerance, making it a relevant target in studies of ubiquitin system dysregulation.

At the molecular level, Ubiquitin C-terminal hydrolase L3 contains a conserved thiol protease catalytic domain characteristic of UCH family enzymes. Its apparent activity in biochemical assays can vary depending on substrate context and cellular conditions rather than changes in protein size or sequence. Ubiquitin C-terminal hydrolase L3 antibody reagents enable investigation of deubiquitination pathways and ubiquitin system regulation, with NSJ Bioreagents providing reagents intended for research use.

UniProt: P15374

Pathways: Feeding Behaviour, Positive Regulation of fat Cell Differentiation