UBXN1 antibody (AA 1-286)

Catalog No. ABIN8093548

This Rabbit Polyclonal antibody specifically detects UBXN1 in WB, ELISA, IP and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for UBXN1 antibody (AA 1-286) (ABIN8093548)

Target: UBXN1 (UBX Domain Protein 1 (UBXN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBXN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Product Details anti-UBXN1 Antibody

Binding Specificity: AA 1-286

Purpose: UBXN1 Antibody / UBX domain-containing protein 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human UBX domain-containing protein 1 recombinant protein (amino acids M1-S286) was used as the immunogen for the UBXN1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the UBXN1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the UBXN1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for UBXN1

Target: UBXN1 (UBX Domain Protein 1 (UBXN1))

Alternative Name: UBXN1

Background: UBXN1 antibody targets UBX domain-containing protein 1, encoded by the UBXN1 gene. UBXN1 is a cytoplasmic and endoplasmic reticulum-associated protein that belongs to the UBX domain protein family, a group of cofactors that interact with the AAA ATPase VCP, also known as p97. Through its UBX domain, UBXN1 participates in protein quality control pathways by linking VCP to specific substrates and adaptor complexes. This positioning allows UBXN1 to influence the fate of misfolded, ubiquitinated, or regulatory proteins within the cell.

Functionally, UBX domain-containing protein 1 is involved in ubiquitin-dependent processes that regulate protein turnover and cellular homeostasis. UBXN1 has been shown to modulate endoplasmic reticulum-associated degradation, a pathway responsible for identifying and removing misfolded proteins from the ER. By coordinating with VCP and other ubiquitin pathway components, UBXN1 helps ensure efficient extraction and downstream handling of these substrates. A UBXN1 antibody supports studies focused on protein degradation pathways and ubiquitin-mediated regulation.

Beyond ER stress responses, UBXN1 also contributes to broader aspects of proteostasis and signaling. It has been implicated in the regulation of inflammatory signaling pathways, where it can influence the stability or activity of signaling intermediates. Through these interactions, UBXN1 acts as a regulatory scaffold rather than an enzymatic factor, fine-tuning cellular responses to stress and signaling cues. These roles place UBXN1 at the intersection of protein quality control and signal transduction.

UBXN1 is expressed in multiple tissues and cell types, reflecting the universal need for regulated protein turnover. Its cellular localization can vary depending on context, with enrichment in cytoplasmic compartments associated with the ER and protein degradation machinery. UBXN1 can associate with additional cofactors beyond VCP, suggesting that it participates in dynamic multiprotein complexes that adapt to changing cellular conditions.

From a disease-relevance perspective, dysregulation of ubiquitin-proteasome and ERAD pathways is linked to neurodegenerative disorders, inflammatory diseases, and cancer. Because UBXN1 modulates these pathways indirectly through adaptor functions, it has attracted interest as a regulatory node rather than a core degradation enzyme. Altered UBXN1 expression or function may influence how cells cope with proteotoxic stress, making it a relevant target for mechanistic studies of disease-associated protein homeostasis.

At the molecular level, UBX domain-containing protein 1 contains a conserved UBX domain that mediates interaction with VCP, along with additional regions that contribute to substrate and adaptor binding. Post-translational modifications and complex assembly state can influence UBXN1 activity and its apparent behavior in biochemical assays without implying changes to the primary amino acid sequence. A UBXN1 antibody is suitable for research applications aimed at investigating protein quality control, ubiquitin-dependent pathways, and stress-responsive cellular mechanisms, with NSJ Bioreagents providing reagents intended for research use.

UniProt: Q04323