Uroplakin 3B antibody (AA 100-300)

Catalog No. ABIN8093557

This Mouse Monoclonal antibody specifically detects Uroplakin 3B in IHC. It exhibits reactivity toward Human.

Quick Overview for Uroplakin 3B antibody (AA 100-300) (ABIN8093557)

Target: Uroplakin 3B (UPK3B)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Uroplakin 3B antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-736M

Product Details anti-Uroplakin 3B Antibody

Binding Specificity: AA 100-300

Purpose: UPK3B Antibody / Uroplakin 3B

Purification: Protein G affinity

Immunogen: A recombinant fragment (around amino acids 100-300) of human Uroplakin 3B (UPK3B) protein (exact sequence is proprietary) was used as the immunogen for the UPK3B antibody for IHC.

Isotype: IgG1, kappa

Application Details

Application Notes: 1. Optimal dilution of the UPK3B antibody for IHC should be determined by the researcher.

2. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: UPK3B/Uroplakin 3B antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for Uroplakin 3B

Target: Uroplakin 3B (UPK3B)

Alternative Name: UPK3B

Background: UPK3B antibody recognizes Uroplakin 3B, a membrane-associated protein encoded by the UPK3B gene and commonly referred to as Uroplakin III beta or UPIII beta. UPK3B Antibody for IHC is developed for immunohistochemical detection of this epithelial differentiation marker in formalin-fixed, paraffin-embedded tissue specimens. Uroplakin 3B localizes predominantly to the plasma membrane of specialized epithelial cells, where it contributes to apical membrane structure and barrier integrity.

UPK3B antibody, also known as Uroplakin III beta antibody, UPIII beta antibody, and Uroplakin IIIb antibody in the literature, targets a member of the uroplakin family involved in formation of asymmetric unit membrane plaques. Although Uroplakin 3A is classically associated with terminally differentiated urothelial umbrella cells, Uroplakin 3B demonstrates a broader expression profile, particularly in mesothelial cells lining the pleura, peritoneum, and pericardium. In these tissues, UPIII beta typically exhibits distinct membranous staining reflecting its cell surface localization.

Structurally, Uroplakin 3B is a single-pass transmembrane glycoprotein containing an extracellular domain, a transmembrane segment, and a short cytoplasmic tail. It forms heterodimeric complexes with other uroplakin family members and participates in stabilization of specialized apical membrane domains. Its membranous distribution supports maintenance of epithelial surface architecture and barrier function in differentiated tissues.

UPK3B expression has been documented in normal mesothelium and in mesothelioma, as well as in certain ovarian and Mullerian-derived tumors. Because of this lineage association, Uroplakin III beta antibody is frequently used in research examining mesothelial differentiation and epithelial tumor classification. Distinct membranous staining patterns are particularly informative when evaluating tissue architecture and distinguishing mesothelial from non-mesothelial neoplasms in IHC-based studies.

The mouse monoclonal clone MSVA-736M targets Uroplakin 3B for immunohistochemical research applications. Visualization of crisp membranous staining supports investigation of mesothelial biology, epithelial differentiation status, and tumor origin in tissue-based analyses at NSJ Bioreagents.

This antibody is also part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.

UniProt: Q9BT76