p107 antibody (pThr369)

Catalog No. ABIN760691

The Rabbit Polyclonal anti-p107 antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect p107 in samples from Human.

Quick Overview for p107 antibody (pThr369) (ABIN760691)

Target: p107 (RBL1) (Retinoblastoma-Like 1 (p107) (RBL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p107 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-p107 Antibody

Binding Specificity: pThr369

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human p107 around the phosphorylation site of Thr369

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for p107

Target: p107 (RBL1) (Retinoblastoma-Like 1 (p107) (RBL1))

Alternative Name: P107

Background:

Synonyms: RBL1phospho T369, 107 kDa retinoblastoma-associated protein, Cellular protein 107, Cellular protein p107, CP107, MGC40006, p107, PRB1, RBL1, RBL1_HUMAN, Retinoblastoma like protein 1, retinoblastoma-like 1 p107, Retinoblastoma-like protein 1.

Background: p107 is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene.

Gene ID: 5933

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases