ACER1 antibody (AA 100-180)

Catalog No. ABIN8119660

The Rabbit Polyclonal anti-ACER1 antibody (ABIN8119660) specifically detects ACER1 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ACER1 antibody (AA 100-180) (ABIN8119660)

Target: ACER1 (Alkaline Ceramidase 1 (ACER1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACER1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ACER1 Antibody

Binding Specificity: AA 100-180

Purpose: ASAH3 Antibody

Specificity: ASAH3 Polyclonal Antibody detects endogenous levels of ASAH3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from ASAH3 . at AA range: 100-180

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for ACER1

Target: ACER1 (Alkaline Ceramidase 1 (ACER1))

Alternative Name: ASAH3

Background:

Synonyms: ACER1, ASAH3, Alkaline ceramidase 1, AlkCDase 1, Alkaline CDase 1, Acylsphingosine deacylase 3, N-acylsphingosine amidohydrolase 3

Background: Ceramides are synthesized during epidermal differentiation and accumulate within the interstices of the stratum corneum, where they represent critical components of the epidermal permeability barrier. Excess cellular ceramide can trigger antimitogenic signals and induce apoptosis, and the ceramide metabolites sphingosine and sphingosine-1-phosphate (S1P) are important bioregulatory molecules. Ceramide hydrolysis in the nucleated cell layers regulates keratinocyte proliferation and apoptosis in response to external stress. Ceramide hydrolysis also occurs at the stratum corneum, releasing free sphingoid base that functions as an endogenous antimicrobial agent. ACER1 is highly expressed in epidermis and catalyzes the hydrolysis of very long chain ceramides to generate sphingosine (Houben et al., 2006 [PubMed 16477081], Sun et al., 2008 [PubMed 17713573]).[supplied by OMIM, Jul 2010],

Gene Name: ACER1

Protein Name: Alkaline ceramidase 1

Gene ID: 125981

UniProt: Q8TDN7