ACLY antibody

Catalog No. ABIN8119859

This Rabbit Monoclonal antibody specifically detects ACLY in WB, ELISA, IHC, IF and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ACLY antibody (ABIN8119859)

Target: ACLY (ATP Citrate Lyase (ACLY))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ACLY antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB2029

Product Details anti-ACLY Antibody

Purpose: ATP-Citrate Lyase Rabbit mAb

Specificity: This antibody detects endogenous levels of ATP-Citrate Lyase protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:2000-1:10000 | IHC, 1:2000-1:10000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for ACLY

Target: ACLY (ATP Citrate Lyase (ACLY))

Alternative Name: ATP-Citrate Lyase

Background:

Synonyms: ACLY, ATP-citrate synthase, ATP-citrate, pro-S-)-lyase, ACL, Citrate cleavage enzyme

Background: ATP citrate lyase (ACLY) Homo sapiens    ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440, 000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014],

Gene Name: ACLY

Protein Name: ATP-Citrate Lyase

Gene ID: 47

UniProt: P53396

Pathways: Warburg Effect