CEP55 antibody (pSer425)

Catalog No. ABIN8120461

The Rabbit Polyclonal anti-CEP55 antibody (ABIN8120461) specifically detects CEP55 in WB, ELISA, IHC and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for CEP55 antibody (pSer425) (ABIN8120461)

Target: CEP55 (Centrosomal Protein 55kDa (CEP55))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEP55 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CEP55 Antibody

Binding Specificity: pSer425

Purpose: CEP55 (phospho Ser425) Antibody

Specificity: Phospho-CEP55 (S425) Polyclonal Antibody detects endogenous levels of CEP55 protein only when phosphorylated at S425.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized phospho-peptide around the phosphorylation site of human CEP55 (phospho Ser425)

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for CEP55

Target: CEP55 (Centrosomal Protein 55kDa (CEP55))

Alternative Name: CEP55

Background:

Synonyms: CEP55, C10orf3, URCC6, Centrosomal protein of 55 kDa, Cep55, Up-regulated in colon cancer 6

Background: function:Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis.,PTM:There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully.,subcellular location:Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loss of affinity for the centrosome at the onset of prophase and diffusion throughout the cell. This dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis.,subunit:Homodimer. Interacts (phosphorylated on Ser-425 and Ser-428) with PLK1. Interacts with AKAP9, the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PCNT, the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PDCD6IP, the interaction is direct, CEP55 binds PDCD6IP in a 2:1 stoechiometry, PDCD6IP competes with TSG101 for the same binding site. Interacts with TSG101, TSG101 competes with PDCD6IP for the same binding site, interaction is required for cytokinesis but not for viral budding. Interacts with FAM125A, VPS37B, VPS37C and VPS28.,tissue specificity:Widely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in adult and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain.,

Gene Name: CEP55

Protein Name: Centrosomal protein of 55 kDa

Gene ID: 55165

UniProt: Q53EZ4