Claudin 1 antibody

Catalog No. ABIN8120591

This Rabbit Monoclonal antibody specifically detects Claudin 1 in WB, IHC, ELISA, IF and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Claudin 1 antibody (ABIN8120591)

Target: Claudin 1 (CLDN1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Claudin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB1862

Product Details anti-Claudin 1 Antibody

Purpose: Claudin 1 Rabbit mAb

Specificity: This antibody detects endogenous levels of Claudin 1 protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:1000-1:5000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for Claudin 1

Target: Claudin 1 (CLDN1)

Alternative Name: Claudin 1

Background:

Synonyms: CLDN1, CLD1, SEMP1, Claudin-1, Senescence-associated epithelial membrane protein

Background: Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008],

Gene Name: CLDN1

Protein Name: Claudin 1

Gene ID: 9076

UniProt: O95832

Pathways: Cell-Cell Junction Organization, Hepatitis C