NEDD4-2 antibody (pSer448)

Catalog No. ABIN8121118

The Rabbit Polyclonal anti-NEDD4-2 antibody (ABIN8121118) specifically detects NEDD4-2 in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NEDD4-2 antibody (pSer448) (ABIN8121118)

Target: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEDD4-2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NEDD4-2 Antibody

Binding Specificity: pSer448

Purpose: NEDD4L (Phospho Ser448) Antibody

Specificity: This antibody detects endogenous levels of Human NEDD4L (phospho-Ser448)

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized phosho peptide around human NEDD4L (Ser448)

Isotype: IgG

Application Details

Application Notes: WB 1:1000-2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for NEDD4-2

Target: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Alternative Name: NEDD4L

Background:

Synonyms: E3 ubiquitin-protein ligase NEDD4-like (EC 6.3.2.-) (NEDD4.2) (Nedd4-2)

Background: This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012],

Gene Name: NEDD4L KIAA0439 NEDL3

Protein Name: NEDD4L (Ser448)

Gene ID: 23327

UniProt: Q96PU5

Pathways: Negative Regulation of Transporter Activity