ERG antibody (AA 200-300)

Catalog No. ABIN8121257

This Mouse Monoclonal antibody specifically detects ERG in WB, ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for ERG antibody (AA 200-300) (ABIN8121257)

Target: ERG (ERG, ETS transcription factor (ERG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: AB2444

Product Details anti-ERG Antibody

Binding Specificity: AA 200-300

Purpose: ERG mouse mAb

Specificity: This antibody detects endogenous levels of ERG protein.

Purification: The antibody was purified by protein G affinity chromatography.

Immunogen: A synthetic peptide corresponding to the amino acid region 200 - 300 of the human ERG protein.

Isotype: IgG

Application Details

Application Notes: WB, 1:500-1:2000 | IF, 1:100-1:500 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for ERG

Target: ERG (ERG, ETS transcription factor (ERG))

Alternative Name: ERG

Background:

Synonyms: Transcriptional regulator ERG, Transforming protein ERG, ERG

Background: This gene encodes a member of the erythroblast transformation-specific (ETS) family of transcriptions factors. All members of this family are key regulators of embryonic development, cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. The protein encoded by this gene is mainly expressed in the nucleus. It contains an ETS DNA-binding domain and a PNT (pointed) domain which is implicated in the self-association of chimeric oncoproteins. This protein is required for platelet adhesion to the subendothelium, inducing vascular cell remodeling. It also regulates hematopoesis, and the differentiation and maturation of megakaryocytic cells. This gene is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia. More than two dozens of transcript variants generated from combinatorial usage of three alternative promoters and multiple alternative splicing events have been reported, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Apr 2014]

Gene Name: ERG

Protein Name: ERG

Gene ID: 2078

UniProt: P11308