EIF4B antibody

Catalog No. ABIN8121318

The Rabbit Monoclonal anti-EIF4B antibody is suitable to detect EIF4B in samples from Human. It has been validated for WB, IHC, ELISA, IF and IP.

Quick Overview for EIF4B antibody (ABIN8121318)

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EIF4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB2201

Product Details anti-EIF4B Antibody

Purpose: eIF4B Rabbit mAb

Specificity: This antibody detects endogenous levels of eIF4B protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:2000-1:10000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for EIF4B

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Alternative Name: eIF4B

Background:

Synonyms: Eukaryotic translation initiation factor 4B, eIF-4B,

Background: cRequired for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. , similarity:Contains 1 RRM (RNA recognition motif) domain. , subunit:Self-associates and interacts with EIF3 p170 subunit.

Gene Name: EIF4B

Protein Name: eIF4B

Gene ID: 1975

UniProt: P23588