GLN1 antibody

Catalog No. ABIN8121699

The Rabbit Monoclonal anti-GLN1 antibody is suitable to detect GLN1 in samples from Human, Rat and Mouse. It has been validated for WB, IHC, IF, IP and ELISA.

Quick Overview for GLN1 antibody (ABIN8121699)

Target: GLN1 (Glutamine Synthetase (GLN1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GLN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA

Clone: AB1655

Product Details anti-GLN1 Antibody

Purpose: Glutamine Synthetase Rabbit mAb

Specificity: This antibody detects endogenous levels of Glutamine Synthetase protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:1000-1:5000 | IHC, 1:1000-1:4000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for GLN1

Target: GLN1 (Glutamine Synthetase (GLN1))

Alternative Name: Glutamine Synthetase

Background:

Synonyms: GLUL, GLNS, Glutamine synthetase, GS, Glutamate decarboxylase, Glutamate-- ammonia ligase

Background: The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014],

Gene Name: >>Arginine biosynthesis, >>Alanine, aspartate and glutamate metabolism, >>Glyoxylate and dicarboxylate metabolism, >>Nitrogen metabolism, >>Metabolic pathways, >>Biosynthesis of amino acids, >>Necroptosis, >>Glutamatergic synapse, >>GABAergic synapse

Protein Name: Glutamine Synthetase

Gene ID: 2752

UniProt: P15104

Pathways: Positive Regulation of Peptide Hormone Secretion