GPX4 antibody (AA 84-134)

Catalog No. ABIN8121711

The Rabbit Polyclonal anti-GPX4 antibody is suitable to detect GPX4 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for GPX4 antibody (AA 84-134) (ABIN8121711)

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPX4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GPX4 Antibody

Binding Specificity: AA 84-134

Purpose: GPX4 Antibody

Specificity: This antibody detects endogenous levels of GPX4 at Human

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human GPX4 AA range: 84-134

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for GPX4

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Alternative Name: GPX4

Background:

Synonyms: Phospholipid hydroperoxide glutathione peroxidase, mitochondrial (PHGPx) (EC 1.11.1.12) (Glutathione peroxidase 4) (GPx-4) (GSHPx-4)

Background: The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2016],

Gene Name: GPX4

Protein Name: GPX4

Gene ID: 2879

UniProt: P36969