GPX4 antibody

Catalog No. ABIN8121712

The Rabbit Monoclonal anti-GPX4 antibody (Clone AB2084) (ABIN8121712) specifically detects GPX4 in WB, ELISA, IF and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for GPX4 antibody (ABIN8121712)

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GPX4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB2084

Product Details anti-GPX4 Antibody

Purpose: GPX4 Rabbit mAb

Specificity: This antibody detects endogenous levels of GPX4 protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:2000-1:10000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for GPX4

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Alternative Name: GPX4

Background:

Synonyms: Phospholipid hydroperoxide glutathione peroxidase, mitochondrial, PHGPx, Glutathione peroxidase 4, GPx-4, GSHPx-4,

Background: The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development, thus, it has been identified as a &apos, moonlighting&apos, protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS) . This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3&apos, UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2016],

Gene Name: GPX4

Protein Name: GPX4

Gene ID: 2879

UniProt: P36969