HCLS1 antibody (pTyr378)

Catalog No. ABIN8121917

This Rabbit Polyclonal antibody specifically detects HCLS1 in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HCLS1 antibody (pTyr378) (ABIN8121917)

Target: HCLS1 (Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCLS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-HCLS1 Antibody

Binding Specificity: pTyr378

Purpose: Lck BP-1 (phospho Tyr378) Antibody

Specificity: Phospho-Lck BP-1 (Y378) Polyclonal Antibody detects endogenous levels of Lck BP-1 protein only when phosphorylated at Y378.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized phospho-peptide around the phosphorylation site of human Lck BP-1 (phospho Tyr378)

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000,IHC 1:50-300, ELISA 2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for HCLS1

Target: HCLS1 (Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1))

Alternative Name: Lck BP-1

Background:

Synonyms: HCLS1, HS1, Hematopoietic lineage cell-specific protein, Hematopoietic cell-specific LYN substrate 1, LckBP1, p75

Background: developmental stage:Expressed in early stage of myeloid and erythroid differentiation.,function:Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.,PTM:Phosphorylated by LYN, rapidly after cross-linking of surface IgM on B-cells.,similarity:Contains 1 SH3 domain.,similarity:Contains 4 cortactin repeats.,subunit:Associates with the SH2 and SH3 domains of LCK. Binding to he LCK SH3 domain occurs constitutively, while binding to the LCK SH2 domain occurs only upon TCR stimulation. A similar binding pattern was observed with LYN, but not with FYN in which the FYN SH2 region associates upon TCR stimulation but the FYN SH3 region does not associate regardless of TCR stimulation. Directly associates with HAX1, through binding to its C-terminal region. Interacts with HS1BP3.,tissue specificity:Expressed only in tissues and cells of hematopoietic origin.,

Gene Name: HCLS1

Protein Name: Hematopoietic lineage cell-specific protein

Gene ID: 3059

UniProt: P14317

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Maintenance of Protein Location