HCAR2 antibody (AA 260-340)

Catalog No. ABIN8122128

The Rabbit Polyclonal anti-HCAR2 antibody is suitable to detect HCAR2 in samples from Human, Mouse and Rat. It has been validated for ELISA and WB.

Quick Overview for HCAR2 antibody (AA 260-340) (ABIN8122128)

Target: HCAR2 (Hydroxycarboxylic Acid Receptor 2 (HCAR2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCAR2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-HCAR2 Antibody

Binding Specificity: AA 260-340

Purpose: HCAR2 Antibody

Specificity: HCAR2 Polyclonal Antibody detects endogenous levels of protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human protein . at AA range: 260-340

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000 ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for HCAR2

Target: HCAR2 (Hydroxycarboxylic Acid Receptor 2 (HCAR2))

Alternative Name: HCAR2

Background:

Background: developmental stage:Expression in neutrophils occurs in the late terminal differentiation phase.,function:Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis.,miscellaneous:The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Expression largely restricted to adipose tissue and spleen. Expressed on mature neutrophils but not on immature neutrophils or eosinophils.,

Gene Name: HCAR2 GPR109A HCA2 HM74A NIACR1

Protein Name: Hydroxycarboxylic acid receptor 2 (G-protein coupled receptor 109A) (G-protein coupled receptor HM74A) (Niacin receptor 1) (Nicotinic acid receptor)

Gene ID: 338442

UniProt: Q8TDS4