MASP1 antibody (Cleaved-Ile449, Light Chain)

Catalog No. ABIN8122756

The Rabbit Polyclonal anti-MASP1 antibody (ABIN8122756) specifically detects MASP1 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MASP1 antibody (Cleaved-Ile449, Light Chain) (ABIN8122756)

Target: MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MASP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MASP1 Antibody

Binding Specificity: Cleaved-Ile449, Light Chain

Purpose: MASP1 (light chain, Cleaved-Ile449) Antibody

Specificity: This antibody detects endogenous levels of Human MASP1 (light chain, Cleaved-Ile449, protein was cleaved amino acid sequence between 448-449 )

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized peptide derived from human MASP1 (light chain, Cleaved-Ile449)

Isotype: IgG

Application Details

Application Notes: WB 1:1000-2000 ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for MASP1

Target: MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))

Alternative Name: MASP1

Background:

Synonyms: Mannan-binding lectin serine protease 1 (EC 3.4.21.-, Complement factor MASP-3, Complement-activating component of Ra-reactive factor, Mannose-binding lectin-associated serine protease 1, MASP-1, Mannose-binding protein-associated serine protease, Ra-reactive factor serine protease p100, RaRF, Serine protease 5) [Cleaved into: Mannan-binding lectin serine protease 1 heavy chain, Mannan-binding lectin serine protease 1 light chain]

Background: enzyme regulation:Inhibited by SERPING1 and A2M.,function:Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5.,PTM:Autoproteolytic processing of the proenzyme produces the active enzyme composed on the heavy and the light chain held together by a disulfide bond. Isoform 1 but not isoform 2 is activated through autoproteolytic processing.,PTM:N-glycosylated. Some N-linked glycan are of the complex-type.,PTM:The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.,similarity:Belongs to the peptidase S1 family.,similarity:Contains 1 EGF-like domain.,similarity:Contains 1 peptidase S1 domain.,similarity:Contains 2 CUB domains.,similarity:Contains 2 Sushi (CCP/SCR) domains.,subunit:Homodimer. Interacts with the oligomeric lectins MBL2, FCN2 and FCN3, triggers the lectin pathway of complement through activation of C3. Interacts with SERPING1.,tissue specificity:Protein of the plasma which is primarily expressed by liver.,

Gene Name: MASP1 CRARF CRARF1 PRSS5

Protein Name: MASP1 (light chain, Cleaved-Ile449)

Gene ID: 5648

UniProt: P48740

Pathways: Complement System