Nestin antibody (AA 400-500)

Catalog No. ABIN8123269

The Mouse Monoclonal anti-Nestin antibody (Clone AB2428) (ABIN8123269) specifically detects Nestin in IHC, WB, IF, ELISA and IP. The antibody is reactive with Human samples.

Quick Overview for Nestin antibody (AA 400-500) (ABIN8123269)

Target: Nestin (NES)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP)

Clone: AB2428

Product Details anti-Nestin Antibody

Binding Specificity: AA 400-500

Purpose: Nestin mouse mAb

Specificity: This antibody detects endogenous levels of Nestin protein.

Purification: The antibody was purified by protein G affinity chromatography.

Immunogen: A synthetic peptide corresponding to the amino acid region 400 - 500 of the human Beta Tubulin protein.

Isotype: IgG

Application Details

Application Notes: IHC, 1:100-1:500 | WB, 1:500-1:2000 | IF, 1:100-1:500 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. | IP, 0. 5 μg-1:4 μg antibody for 200 μg-1:400 μg extracts of whole cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin

Background:

Synonyms: Nestin

Background: This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011],

Gene Name: NES Nbla00170

Protein Name: Nestin

Gene ID: 10763

UniProt: P48681