NEFL antibody (AA 400-543)

Catalog No. ABIN8123294

This Mouse Monoclonal antibody specifically detects NEFL in WB, IHC, IF and ELISA. It exhibits reactivity toward Human.

Quick Overview for NEFL antibody (AA 400-543) (ABIN8123294)

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Clone: AB1421

Product Details anti-NEFL Antibody

Binding Specificity: AA 400-543

Purpose: Neurofilament-L mouse mAb

Specificity: The antibody can specifically recognize human Neurofilament protein, especilaly NF-L protein.

Purification: The antibody was purified by protein G affinity chromatography.

Immunogen: A synthetic peptide corresponding to the amino acid region 400 - 543 of the human Neurofilament protein.

Isotype: IgG

Application Details

Application Notes: IHC, 1:200-1:1000 | WB, 1:500-1:2000 | IF, 1:100-1:500 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for NEFL

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Alternative Name: Neurofilament-L

Background:

Synonyms: 150 kDa medium, 160 kDa neurofilament protein, 2 kDa neurofilament protein, 200 kDa neurofilament protein, 68 kDa neurofilament protein, AI847934, CMT1F, CMT2CC, CMT2E, FLJ53642, KIAA0845, light molecular weight neurofilament protein, Micro glutamic acid-rich protein, mKIAA0845, Nef3, NEFH, NEFL, NEFM, Neurofilament 3, Neurofilament heavy polypeptide 200 kDa, Neurofilament heavy polypeptide, Neurofilament light polypeptide 68 kDa, Neurofilament light polypeptide, Neurofilament medium polypeptide 150 kDa, Neurofilament medium polypeptide, Neurofilament protein heavy polypeptide, Neurofilament protein light chain, Neurofilament protein light polypeptide, Neurofilament protein medium polypeptide, neurofilament subunit NF-L, Neurofilament triplet H protein, Neurofilament triplet L protein, Neurofilament triplet M protein, neurofilament-3 (150 kD medium) , NF-H, NF-L, NF-M, NF160, NF165, NF200, NF68, NFH, NFL, NFM, NFM_HUMAN, PPP1R110, protein phosphatase 1, regulatory subunit 110

Background: Neurofilament (NF) is a neuron specific intermediate filament protein. It is a polymer composed of subunits of NF-Human, Rat, Mouse, nd NF-L. It exists in neurons, neurites, peripheral nerve fibers, sympathetic ganglion cells and adrenal medulla. It is expressed in ganglioneuroma, paraganglioma, cerebellar or peripheral neuroblastoma, adrenal tumor or peripheral pheochromocytoma. It is helpful to the diagnosis and differential diagnosis of neuroblastoma and pheochromocytoma, and to identify the neurons of Hirschsprung's disease.

Gene Name: NEFL

Protein Name: Neurofilament

Gene ID: 4747

UniProt: P07196