NEFL antibody (AA 400-543)

Catalog No. ABIN8123295

The Mouse Monoclonal anti-NEFL antibody is suitable to detect NEFL in samples from Human, Rat and Mouse. It has been validated for WB, IHC, IF and ELISA.

Quick Overview for NEFL antibody (AA 400-543) (ABIN8123295)

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Clone: AB1438

Product Details anti-NEFL Antibody

Binding Specificity: AA 400-543

Purpose: Neurofilament-L mouse mAb

Specificity: The antibody can specifically recognize human Neurofilament protein, especilaly NF-L protein.

Purification: The antibody was purified by protein G affinity chromatography.

Immunogen: A synthetic peptide corresponding to the amino acid region 400 - 543 of the human Neurofilament protein.

Isotype: IgG

Application Details

Application Notes: IHC, 1:200-1:1000 | WB, 1:500-1:2000 | IF, 1:100-1:500 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for NEFL

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Alternative Name: Neurofilament-L

Background:

Synonyms: Neurofilament light polypeptide (NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein)

Background: Neurofilament (NF) is a neuron specific intermediate filament protein. It is a polymer composed of subunits of NF-Human, Rat, Mouse, nd NF-L. It exists in neurons, neurites, peripheral nerve fibers, sympathetic ganglion cells and adrenal medulla. It is expressed in ganglioneuroma, paraganglioma, cerebellar or peripheral neuroblastoma, adrenal tumor or peripheral pheochromocytoma. It is helpful to the diagnosis and differential diagnosis of neuroblastoma and pheochromocytoma, and to identify the neurons of Hirschsprung's disease.

Gene Name: NEFL NF68 NFL

Protein Name: Neurofilament

Gene ID: 4747

UniProt: P07196