NRF1 antibody

Catalog No. ABIN8123448

The Rabbit Monoclonal anti-NRF1 antibody is suitable to detect NRF1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA, IF and IP.

Quick Overview for NRF1 antibody (ABIN8123448)

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NRF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB2165

Product Details anti-NRF1 Antibody

Purpose: Nrf1 Rabbit mAb

Specificity: This antibody detects endogenous levels of Nrf1 protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:2000-1:10000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for NRF1

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Alternative Name: Nrf1

Background:

Synonyms: NRF1, Nuclear respiratory factor 1, NRF-1, Alpha palindromic-binding protein, Alpha-pal

Background: This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for ", nuclear factor (erythroid-derived 2)-like 1", which has an official symbol of NFE2L1. [provided by RefSeq, May 2014],

Gene Name: NRF1

Protein Name: Nrf1

Gene ID: 4899

UniProt: Q16656

Pathways: Regulation of Lipid Metabolism by PPARalpha