Nucleolin antibody

Catalog No. ABIN8123459

This Rabbit Monoclonal antibody specifically detects Nucleolin in WB, IHC, IF, ELISA and IP. It exhibits reactivity toward Human and Mouse.

Quick Overview for Nucleolin antibody (ABIN8123459)

Target: Nucleolin (NCL)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP)

Clone: AB1687

Product Details anti-Nucleolin Antibody

Purpose: Nucleolin Rabbit mAb

Specificity: This antibody detects endogenous levels of Nucleolin protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:500-1:2000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background:

Synonyms: Nucleolin (Protein C23)

Background: Nucleolin (NCL) , a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008],

Gene Name: NCL

Protein Name: Nucleolin

Gene ID: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization