POLR1B antibody

Catalog No. ABIN8123864

This Rabbit Polyclonal antibody specifically detects POLR1B in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for POLR1B antibody (ABIN8123864)

Target: POLR1B (Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-POLR1B Antibody

Purpose: RPA135 Antibody

Specificity: RPA135 Polyclonal Antibody detects endogenous levels of RPA135 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human RPA135.

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for POLR1B

Target: POLR1B (Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B))

Alternative Name: RPA135

Background:

Synonyms: POLR1B, DNA-directed RNA polymerase I subunit RPA2, RNA polymerase I subunit 2, DNA-directed RNA polymerase I 135 kDa polypeptide, RPA135

Background: Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008],

Gene Name: POLR1B

Protein Name: DNA-directed RNA polymerase I subunit RPA2

Gene ID: 84172

UniProt: Q9H9Y6